葛根素对胰岛素抵抗大鼠脂肪组织中糖原合成酶激酶 3β表达的影响

目的: 探讨葛根素对高脂饮食诱导的胰岛素抵抗大鼠脂肪组织中糖原合成酶激酶 3β(GSK-3β)表达的影响 。方法: 30 只 SPF 级雄性 Wister大鼠随机分为2 组,正常对照组 10只,常规饲料喂养,模型组20 只,高脂饲料喂养;4 周后模型形成,模型组随机分为2 组(胰岛素抵抗组 、葛根素干预组,每组 10只),继以高脂饲料喂养,葛根素干预组同时给予葛根素注射液 100 mg·kg^-1·d^-1 腹腔注射。干预 6 周后,用 Western-blot 方法检测各组大鼠附睾脂肪组织中GSK-3β的表达,分析对比各组的表达差异;定期检测大鼠体重、空腹血糖及血浆胰岛素 、血甘油三酯和胆固醇,并计算胰岛素敏感指数 。结果: 高脂饲料喂养 4周后,与正常对照组比较,模型组大鼠体重(BW) 、空腹血糖 (FPG) 、胰岛素 (FINS) 、胆 固醇(TC) 、甘油三酯(TG) 显著升高(P<0.05 或 P<0.01),胰岛素敏感指数 ISI 显著下降(P<0.01),胰岛素抵抗模型诱导成功;葛根素干预6 周后,与胰岛素抵抗组大鼠比较,脂肪组织中GSK-3β的表达显著下降(P<0.05),和正常对照组比较,无显著差异 。结论: 葛根素显著下调脂肪组织 GSK-3β的表达,并且可能参与其改善胰岛素抵抗的机制。

Effect of puerarin on glucogen synthase Kinase-3 bate in adipocyte of rats with insulin resistance

AIM: To investigate the effect of puerarin on expression of the glucogen synthase kinase-3 bate(GSK-3β) in adipocyte of rats with insulin resistance in-duced by diets.METHODS: 30 Wisterrats were ran-domized into two groups:control grouP(n =10) and model grouP(n =20).Control groupwere fed with gener-al diets,while model grouPwere fed with the high fat di-ets to induce insulin resistance.After4weeks,the mode-ls have formed then were randomized into two groups:model grouP(n =10) and pueraringrouP(n =10).Pu-erarin grouPwere treated with puerarin (100mg·kg^-1·d^-1,ip) for6 weeks.GSK-3βprotein was de-tected by Western-blot analysis.Body weight,serum trig-lyceride and cholesterol,fasting plasma glucose and serum insulin concentration were detected termly and the insulin sensitivity index was calculated.RESULTS: The model grouPshowed hyperglycemia,hyperinsulinism,obviously obesity and the insulin resistance index (ISI) decrease by high fat diets,insulin resistance was induced by this way.Compared with model group,GSK-3βprotein in ad-ipocyte of puerarin grouPrats decreased (P<0.05).CONCLUSION: Puerarin can decrease the expression of GSK-3βprotein in adipocyte of ratswith insulin resistance which may be involved in the mechanism of amelioration the state of insulin resistance.