血管紧张素-(1-7) 对血管紧张素II致人脐静脉内皮细胞损伤的保护作用研究

目的:观察血管紧张素-(1-7) Ang-(1-7)] 对血管紧张素II(Ang II) 致人脐静脉内皮细胞(HUVECs)损伤的保护作用。方法:体外培养的HUVECs随机分为4 组:对照组, AngII 组, Ang-(1-7) 组, AngII+Ang-(1-7) 组。采用分光光度计测定培养的HUVECs乳酸脱氢酶(LDH) 漏出;流式细胞仪检测细胞凋亡;硝酸还原酶法和放射免疫分析技术分别测定HUVECs 上清液中一氧化氮(NO) 和内皮素-1(ET-1)的含量。结果:与对照组比较,Ang II(0.1 μmol°L^-1)显著增加HUVECs LDH 漏出(P<0.01)、ET-1 分泌(P<0.01) 和HUVECs 凋亡率(P<0.01), 显著减少NO 的含量(P<0.05);Ang-(1-7) 呈剂量依赖性抑制了Ang II 的促LDH 漏出、ET-1 分泌、增加细胞凋亡等作用, 同时明显促进HUVECs 的NO 释放;单用Ang-(1-7) 对HUVECs 无明显影响。结论:Ang-(1-7)可抑制Ang II 所致的体外培养HUVECs 损伤, 对内皮细胞具有保护作用。

Protective effect of angiotensin-(1-7) on human umbilical vein endothelial cells injury induced by angiotensin II in culture

AIM: To investigate the protective effects of angiotensin-(1-7) Ang-(1-7)] on human umbilical vein endothelial cells (HUVECs) injury induced by angiotensin II (Ang II) in culture.METHODS: Cultured human umbilical vein endothelial cells were randomly divided into 4 groups:control, Ang II, Ang-(1-7), and AngII+Ang-(1-7).Spectrophotometer and flow cytometry were used to evaluate lactate dehydrogenase (LDH) leakage content and apoptosis percentage, respectively. Nitric oxide (NO) and endothelin-1 (ET-1) contents were measured by colorimetry and radioimmunoassay.RESULTS: 0.1 μmol°L^-1 Ang II significantly increased LDH leakage (P<0.01), ET-1 release (P<0.01), and apoptosis percentage (P<0.01).These increases were inhibited by Ang-(1-7) in a dose-dependent manner, while NO release by HUVECs was promoted by Ang-(1-7).Ang-(1-7) alone had no effect on HUVECs. CONCLUSION: Ang-(1-7) has a protective effect on HUVECs via inhibition of Ang II-induced injury and apoptosis, suggesting that Ang-(1-7) may play an important role in prevention and treatment of vascular diseases.