硫喷妥钠或异丙酚麻醉不同时期大鼠脑ATP 酶和NO 合酶活性以及NO 生成量的动态变化

目的:观察硫喷妥钠或异丙酚麻醉不同时期,大鼠皮层、脑干Ca²⁺-ATPase、Na⁺, K⁺-ATPase 活性、NOS 活性和NO 生成量的动态变化。方法:大鼠腹腔注射硫喷妥钠30 mg°kg^-1 或异丙酚100 mg°kg^-1,以翻正反射消失为标准, 分别于诱导期、麻醉期, 恢复期和苏醒期断头取脑(n=8), 分离出皮层和脑干, 测定Ca²⁺-ATPase、Na⁺, K⁺-ATPase、NOS 的活性以及NO 的生成量, 腹腔注射生理盐水10 ml°kg^-1作为对照组。结果:大鼠皮层、脑干Ca²⁺-ATPase、Na⁺, K⁺-ATPase 的活性在硫喷妥钠诱导期、麻醉期和恢复期显著性降低(P<0.05 或P<0.01), 在异丙酚麻醉期亦显著性降低(P<0.05)。腹腔注射硫喷妥钠或异丙酚后, NOS 活性和NO 生成量从诱导期到恢复期均显著性降低(P<0.01), 在苏醒期可恢复至对照水平。结论:Ca²⁺-ATPase、Na⁺, K⁺-ATPase、NOS 活性及NO 的产生介导了硫喷妥钠或异丙酚的麻醉作用。

Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia

AIM: To investigate the dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases using thiopental and propofol anesthesia.METHODS: Thiopental of 30 mg°kg^-1 or propofol of 100 mg°kg^-1 was administered respectively at different anesthetic phases (induction, anesthesia, restoration, and awake), the activities of NOS and ATPase and NO production in cortex and brain stem were measured.Saline 10 ml°kg^-1 was injected as the control group.RESULTS: Ca²⁺-ATPase and Na⁺, K⁺-ATPase activities in the cortex and brain stem were significantly decreased after administration of thiopental and propofol, especially at induction, anesthesia, or even restoration phase of thiopental group (P<0.05, P<0.01) and at anesthesia phase of propofol group (P<0.05).NOS activities and NO production decreased from induction to restoration phase with thiopental and propofol anesthesia (P<0.01).The parameters were returned near to the normal at awaken phase.CONCLUSION: Activities of ATPases and NOS and the production of NO may mediate the anesthesia effects of thiopental and propofol in the rat cortex and brain stem.