产ESBLs 并高产AmpC 酶肺炎克雷伯氏杆菌中整合子的基因研究

目的:观察4 株从临床分离产超广谱内β 内酰胺酶(ESBLs) 及AmpC 酶肺炎克雷伯氏杆菌的多重耐药情况, 分析其中整合子的存在, 对整合子的特性进行研究, 探讨整合子基因盒表达对肺炎克雷伯杆菌耐药表型的影响。方法:采用微量稀释法测定复方新诺明等15 种抗菌药物对细菌的最低抑菌浓度(MIC) 。PCR 技术检测整合子基因, DNA 测序研究整合子插入耐药基因盒情况。结果:这4 株菌对多种抗菌素耐药。其中1 株存在整合子(扩增片段2 000 bp 左右), 携有dhfrXII 和aadA2 基因。结论:4 株菌中仅1 株存在整合子结构, 尽管产ESBLs和AmpC 酶基因未位于整合子的基因盒上, 但整合子参与多重耐药的产生。

Study of Klebsiella pneumoniae on integron gene of ESBLs and AmpCproducing strains

AIM: To observe multidrug resistance character of ESBLs and AmpC-producing isolated form Klebsiella pneumoniae in clinic, to analyze the existence and character of integron and to investigate the express of gene box involve drug resistance phenotypic.METHODS: MIC of the 4 strains was examined by micro-dilution method.Integron gene was determined by PCR assay with integron specific primer, and DNA sequencing was used for analyzing resistance related gene cassette.RESULTS: The 4 strains were multidrug resistance.The integron was detected in one strain of them, which carried aadA2 and dhfrXII gene.CONCLUSION: Though there is not the genes box of producing ESBLs and AmpC, integron involves in the form of multidrug resistance.