Mn2+对庆大霉素导致的人肾小管上皮细胞系损伤的保护作用

目的: 观察Mn²⁺ 对庆大霉素导致的人肾小管上皮细胞系的损伤有保护作用,并探讨其机制 。方法: 用庆大霉素作用于人肾小管上皮细胞系(HK^-2),造成肾损伤模型,MTT 法检测 Mn²⁺ 对细胞增殖活力的影响;分光光度法检测 Mn²⁺ 导致的 SOD、LDH 活力和 NAG 酶含量的改变;电镜观察 Mn²⁺ 作用后 HK-2 细胞微结构改变。结果: Mn²⁺ 可使庆大霉素损伤的 HK-2 细胞增殖活力增加,降低 LDH 酶活力,减少NAG 酶含量,升高SOD 酶活性,减轻细胞线粒体肿胀程度和减少溶酶体膜的破裂 。结论: Mn²⁺ 对庆大霉素导致的人肾小管上皮细胞系的损伤有保护作用。其机制可能与减轻细胞氧化损伤 、降低线粒体肿胀程度、保护溶酶体的完整性和减少溶酶漏出有关。

Protection of Mn2+on Injury of human kidney tubularepithelial cell line induced by bentamicin

AIM: To evaluate the protection of Mn²⁺ on injury of human kidney tubularepithelial cell line in-duced by gentamicin,and investigate its principals.METHODS: Gentamicin was used to injury human kid-ney tubularepithelial cell line (HK-2) to produce model of kidney injury.MTT method was used to measure effect of Mn2+ on proliferation of cells afterthey were injured by gentamicin.The spectrophotometry method was used to observe the change of LDH,NAG and SOD effected by Mn²⁺.Ultrastructure was examined by electron microsco-py.RESULTS: Although HK-2 cells were injured,Mn²⁺ promoted cells proliferation,decreased LDH activity and NAG content,increased SOD activity,and alleviated dilation of the mitochondria and crushing of lysosomes.CONCLUSION: Mn²⁺ can protect human kidney tubularepithelial cell line from injury induced by gentamicin.It may be in relation to inhibiting the oxidative injury,light-ening dilation of the mitochondria,protecting the integrity of lysosomes and decrease the leakage of enzyme.