| 重离子辐照对红酵母的诱变作用 |
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| 引用本文： | 李红玉,李成华,丁新春,王菊芳,周光明,颉红梅,李强,党秉荣,温小琼,李文建,卫增泉.重离子辐照对红酵母的诱变作用[J].辐射研究与辐射工艺学报,2004,22(1):56-60. |
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| 作者姓名： | 李红玉 李成华 丁新春 王菊芳 周光明 颉红梅 李强 党秉荣 温小琼 李文建 卫增泉 |
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| 作者单位： | 兰州大学生命科学学院,兰州,730000;中国科学院近代物理研究所,兰州,730000 |
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| 基金项目： | 中国科学院“西部之光”(XB98064)资助 |
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| 摘 要： | 应用重离子加速器的50MeV／u^12C^6 重离子对胡萝卜素生产菌-红酵母(Rhodotorula RY Strain)进行辐照处理，经酵母发酵实验，发现50MeV／u^12C^6 重离子对胡萝卜素生产菌-红酵母具有诱变作用。初步筛选到了胡萝卜素产量有变化的辐照变异菌株，并对这些辐照变异菌株进行了RFLP(限制性片段长度多态性)和RAPD(随机扩增DNA多态性)分析，这些工作为工业上利用重离子对胡萝卜素生产菌进行诱变育种展现了新的前景。
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| 关 键 词： | 重离子 红酵母 诱变作用 RFLP RAPD |
| 修稿时间： | 2002年10月25 |
A function of mutagensis on rhodotorula RY strain irradiated by heavy ion |
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| LI Hongyu LI Chenghua DING Xinchun WANG Jufang ZHOU Guangming XIE Hongmei,LI Qiang DANG Bingrong WEN Xiaoqiong LI Wenjian WEI Zengquan.A function of mutagensis on rhodotorula RY strain irradiated by heavy ion[J].Journal of Radiation Research and Radiation Processing,2004,22(1):56-60. |
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| Authors: | LI Hongyu LI Chenghua DING Xinchun WANG Jufang ZHOU Guangming XIE Hongmei LI Qiang DANG Bingrong WEN Xiaoqiong LI Wenjian WEI Zengquan |
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| Affiliation: | LI Hongyu1 LI Chenghua1 DING Xinchun1 WANG Jufang2 ZHOU Guangming2 XIE Hongmei2 LI Qiang2 DANG Bingrong2 WEN Xiaoqiong2 LI Wenjian2 WEI Zengquan2 1 |
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| Abstract: | In this paper, red yeast (Rhodotorula RY Strain) that produces carotene is irradiated by 50MeV/u12C6 heavy ion from Heavy Ion Accelerator in IMP. Fermentation tests show that 50MeV/u12C6 heavy ion has a mutagenesis effect on the red yeast. Some strains of red yeast with changed production of carotene were found by screening. Meanwhile, by RFLP and RAPD analysis, we have a further evidence that heavy ion can cause mutagenesis in Rhodotorula RY Strain. This presents a new prospect for the mutagenesis breeding by heavy ion in industry. |
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| Keywords: | Heavy ion Rhodotorula RY Strain Mutagenesis effect RFLP RAPD |
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