| 含青霉素酰化酶的重组大肠杆菌的发酵培养 |
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| 引用本文： | 杨海波,虞星炬,曲天明,苏志国.含青霉素酰化酶的重组大肠杆菌的发酵培养[J].化学反应工程与工艺,1999,15(3):333-336. |
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| 作者姓名： | 杨海波 虞星炬 曲天明 苏志国 |
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| 作者单位： | 1. 中科院大连化物所,辽宁,大连,116023
2. 中科院化冶所,北京,100080 |
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| 摘 要： | 研究了含青霉素酰化酶的重组菌 Ｅ．ｃｏｌｉ Ａ５６ （ｐ Ｐ Ａ２２）发酵过程中环境因素对酶活的影响，考察了发酵液性质变化与酶活表达之间的关系，获得了酶活较高时的优化环境条件。结果表明，青霉素酰化酶在重组菌生长的平衡期后开始大量表达，苯乙酸作为限制性底物对青霉素酰化酶的合成起诱导作用
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| 关 键 词： | 青霉素酰化酶 发酵培养 |
| 修稿时间： | 1998-11-10 |
FERMENTATION CULTURE OF RECOMBINANT OF E.coli WITH PENICILLIN ACYLASE |
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| YANG Hai-bo,YU Xing-ju,QU Tian-ming,SU Zhi-guo.FERMENTATION CULTURE OF RECOMBINANT OF E.coli WITH PENICILLIN ACYLASE[J].Chemical Reaction Engineering and Technology,1999,15(3):333-336. |
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| Authors: | YANG Hai-bo YU Xing-ju QU Tian-ming SU Zhi-guo |
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| Abstract: | The effects of environm ental factors on the enzym e activity of recom binant E.coli A56(p P A22) w ith penicillin acylase w ere studied during the ferm entation. The rela tionship betw een the characters offerm entation broth and the enzym e activity w as investi gated,and the optim al environm ental conditions for the higher enzym e activity w ere ob tained as w ell. The results show ed thatpenicillin acylase produced w ith a greatam ountaf ter the stationary phase ,and that phenylacetic acid as a lim iting substrate played a role ofinducing the synthesis of penicillin acylase. |
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| Keywords: | penicillin acylase ferm entation culture |
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