| 响应面法优化以重组大肠杆菌发酵生产类人胶原蛋白过程 |
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| 引用本文： | 张驰,范代娣,尚龙安,马晓轩,骆艳娥,薛文娇,高鹏飞.响应面法优化以重组大肠杆菌发酵生产类人胶原蛋白过程[J].中国化学工程学报,2010,18(1):137-142. |
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| 作者姓名： | 张驰 范代娣 尚龙安 马晓轩 骆艳娥 薛文娇 高鹏飞 |
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| 作者单位： | |
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| 基金项目： | 国家自然科学基金，the National High Technology Research and De-velopment Program of China，the Special Research Program of the Education Department of Shaanxi Province |
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| 摘 要： | In order to improve the production of human-like collagen Ⅲ (HLC Ⅲ) by fed-batch culture of recom-binant Escherichia coli BL21, the Plackett-Burman and Box-Behnken design were applied to optimize the fermen-tation process parameters.Three variables (induction time, inoculum age and pH), which have significant effects on HLC Ⅲ production, were selected from eight variables by Plackett-Burman design.With the regression coefficient analysis in the Box-Behnken design, a relationship between HLC Ⅲ production and three significant factors was obtained, and the optimum levels of the three variables were as follows: induction time 3.2h, inoculum age 12.6 h and pH 6.7.The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant.An aver-age 9.68 g·L~(-1)HLC Ⅲ production was attained in the validation experiment under optimized condition, which was 80% higher than the yield of 5.36 g·L~(-1) before optimization.
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| 关 键 词： | human-like collagen Ⅲ |
| 收稿时间： | 2009-3-28 |
| 修稿时间： | 2009-3-28
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Optimization of fermentation process for human-like collagen production of recombinant Escherichia coli using response surface methodology |
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| ZHANG Chi,FAN Daidi,SHANG Long''an,MA Xiaoxuan,LUO Yan''e,XUE Wenjiao,GAO Pengfei.Optimization of fermentation process for human-like collagen production of recombinant Escherichia coli using response surface methodology[J].Chinese Journal of Chemical Engineering,2010,18(1):137-142. |
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| Authors: | ZHANG Chi FAN Daidi SHANG Long'an MA Xiaoxuan LUO Yan'e XUE Wenjiao GAO Pengfei |
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| Affiliation: | 1. School of Life Science, Northwest University, Xi''an 710069, China;2. Shaanxi R&D Center of Biomaterial and Fermentation, Xi''an 710069, China;3. Shaanxi Key Laboratory of Degradable Biomedical Materials, Xi''an 710069, China;4. School of Chemical Engineering, Northwest University, Xi''an 710069, China;5. College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China |
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| Abstract: | In order to improve the production of human-like collagen III (HLC III) by fed-batch culture of recombinant Escherichia coli BL21, the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters. Three variables (induction time, inoculum age and pH), which have significant effects on HLC III production, were selected from eight variables by Plackett-Burman design. With the regression coefficient analysis in the Box-Behnken design, a relationship between HLC III production and three significant factors was obtained, and the optimum levels of the three variables were as follows: induction time 3.2h, inoculum age 12.6 h and pH 6.7. The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant. An average 9.68 g·L?1HLC III production was attained in the validation experiment under optimized condition, which was 80% higher than the yield of 5.36 g·L?1 before optimization. |
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| Keywords: | fermentation process parameters optimization response surface methodology |
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