Esterase activity inspired selection and characterization of zearalenone degrading bacteria Bacillus pumilus ES-21 |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, China;2. Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Beijing 100081, China;3. Department of Food Science, Shaanxi University of Science Technology, Xian 710021, China;4. Department of Environmental Health Science, The University of Georgia, Athens, GA 30602, United States;1. Department of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, Thailand;2. Phileo Lesaffre Animal Care, 278, avenue de la marne, 59700 Marcq-en-Baroeul, France;3. Laboratory Chemical Engineering, University of Toulouse, UMR-CNRS/INPT/UPS 5503, 1 avenue agrobiopôle, 31320 Auzeville-Tolosane, France;1. College of Food Science, Northeast Agricultural University, Harbin 150030, China;2. Laboratory of Agro-product Quality and Safety Risk Assessment (Harbin), Harbin 150086, China;3. Agricultural Products Quality and Safety Research Institute, Heilongjiang Academy of Agriculture, Harbin 150086, China;1. State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu 214122, China;2. Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Beijing 100081, China |
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| Abstract: | Zearalenone (ZEN), mainly produced by Fusarium species, is an estrogenic mycotoxin which causes reproductive disorders in livestock. In this study, we described a simple and rapid method for screening of ZEN-degrading bacteria by esterase activity assay. Soil bacteria strains were first tested for their esterase activities, then active strains were further evaluated for their ZEN-degrading potentials. A bacterial strain named Bacillus pumilus ES-21 was detected to be able to eliminate ZEN in the culture medium. ZEN degradation conditions were optimized through response surface methodology and the result showed that the degradation rate of ZEN by Bacillus pumilus ES-21 was up to 95.7% at the ZEN concentration of 17.9 μg/ml within 24 h. One of the degradation product was proposed to be 1-(3,5-dihydroxyphenyl)-6′-hydroxy-l′-undecen-l0′-one according to LC-TOF-MS/MS analysis. This study provided a strategy for the isolation of ZEN degrading microbes and a promising degrading strain. |
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| Keywords: | Zearalenone Biodegradation Esterase activity |
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