首页 | 本学科首页   官方微博 | 高级检索  
     


A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug-Loaded Nanofibrous Patches
Authors:Sumeyye Cesur  Elif Ilhan  Esra Pilavci  Rabia Betul Sulutas  Merve Gurboga  Ozlem Bingol Ozakpinar  Elif Kaya  Marcin Heljak  Gulgun Bosgelmez Tinaz  Faik Nuzhet Oktar  Oguzhan Gunduz  Ewa Kijeńska-Gawrońska
Affiliation:1. Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul, 34722 Turkey;2. Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul, 34722 Turkey

Department of Bioengineering, Faculty of Engineering, Marmara University, Istanbul, 34854 Turkey;3. Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul, 34722 Turkey

Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul, 34722 Turkey;4. Department of Biochemistry, Faculty of Pharmacy, Marmara University, Istanbul, 34668 Turkey;5. Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Marmara University, Istanbul, 34668 Turkey;6. Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, 02-507 Poland

Abstract:Corneal ulcer, which is brought on by a breach in the epithelial barrier, is a dangerous infection of the avascular corneal stroma. New treatment strategies are needed, suppressing the aggressive nature of the disease and including a combination of different drugs. In this study, vancomycin (VAN) and fluconazole (FLU) dual-drug loaded dual-layered polyvinyl alcohol and gelatin (PVA/GEL) nanofibrous patches are produced by electrospinning. Scanning electron microscopy (SEM) images show smooth surfaces are obtained for both pure and drug-loaded nanofibrous patches. The tensile test results report that loading the FLU and VAN separately into the PVA/GEL patches decrease both the tensile strength and elongation at break and it is further reduced when combining two drug-loaded layers in one patch. According to drug release results, the FLU and VAN-loaded nanofibrous patches show a controlled release profile extending up to 96 h. Moreover, PVA/GEL/FLU, PVA/GEL/VAN, and PVA/GEL/FLU/VAN nanofibrous patches display significant antimicrobial activity against Candida albicans and Staphylococcus aureus. SEM, 4'-6diamidynofenyloindol (DAPI) staining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay show that PVA/FLU and PVA/GEL/FLU/VAN nanofibrous patches have a superior effect on NIH3T3 cell spreading and proliferation. The novelty of this study lays in the development of a potential dual drug rapid treatment for corneal ulcers of aggressive nature.
Keywords:corneal tissue engineering  corneal ulcers  electrospinning  fluconazole  vancomycin
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号