Application of bacteriocin-producing Enterococcus faecium isolated from donkey milk,in the bio-control of Listeria monocytogenes in fresh whey cheese |
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| Affiliation: | 1. Department of Agricultural Biotechnology Center for Food and Bioconvergence, Seoul National University, Seoul 151742, Republic of Korea;2. Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea;3. Innovation LAB, SPC Group, Seoul 137-887, Republic of Korea;4. Research Institute of Food and Biotechnology, SPC Group, Seoul 151-742, Republic of Korea;1. Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China;2. Key Laboratory of Animal Nutrition and Feed Science (Hua Dong), Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | A total of 79 bacterial strains, previously isolated from donkey milk, were screened for their antimicrobial activity against several spoilage and foodborne pathogenic bacteria. Amongst them, 3 strains belonging to Enterococcus faecium displayed antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus and Bacillus cereus. Mass spectrometry analysis demonstrated that all enterococci used in this study produced peptides with masses consistent with those for enterocins A and B. The cell-free supernatants of the identified bacteriocin-producing enterococci were equally active over a wide range of pH and heat treatments making them excellent candidates for potential applications in bio-preservation. Bacteriocins produced by these strains were tested for their capability to control post-processing contamination and growth of L. monocytogenes during refrigerated storage of artificially contaminated fresh whey cheese. One strain was considered bactericidal while the other two were classified as bacteriostatic. |
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