A 12-month longitudinal study of Listeria monocytogenes contamination and persistence in pork retail markets in China |
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| Affiliation: | 1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China;2. Zigong Center for Disease Control and Prevention, Zigong 643000, Sichuan Province, China;3. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia;1. Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality, Ministry of Agriculture of China, Yangzhou University, China;2. Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, China;3. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, China;1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China;2. Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, 510070, PR China;3. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, 510070, PR China;1. National Reference Centre for Emerging Risk in Food Safety, Istituto Zooprofilattico Sperimentale della Lombardia e dell''Emilia Romagna “B. Ubertini”, Brescia, Italy;2. Department of Food Science, University of Parma, Parco Area delle Scienze 49/A, 43124, Parma, Italy;3. Department of Food Microbiology, Istituto Zooprofilattico Sperimentale della Lombardia e dell''Emilia Romagna “B. Ubertini”, Brescia, Italy |
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| Abstract: | Listeria monocytogenes is a foodborne pathogen frequently isolated from raw pork meat. This study was designed to investigate the prevalence and molecular characteristics of L. monocytogenes in raw pork from open markets in China. The survey was conducted monthly over a 12-month period in Zigong, China. L. monocytogenes was isolated from 262 of 1641 samples collected (16.0%) including minced meat samples (131/608, 21.5%), pork pieces samples (111/857, 13.0%) and environmental swabs (20/176, 11.4%). The isolation rates in spring and winter were significantly higher than those in summer and autumn (X2 = 68.85, P < 0.05). All isolates were subjected to serotyping, multi-locus sequence typing (MLST) and AscI pulsed-field gel electrophoresis (PFGE). The 262 isolates were subtyped into five serotypes: 1/2b (43.1%), 1/2c (35.5%), 1/2a (19.1%), 4b (1.1%), 3a (1.1%); 20 sequence types (STs) with four most frequent STs, being ST9 (35.9%), ST87 (19.8%), ST3 (16.0%) and ST8 (14.1%); and 39 pulsotypes (PTs) with PT4 (26.3%), PT30 (14.5%) and PT11 (12.6%) being most frequent. Two primary pulsotypes from pork pieces were previously isolated from clinical listeriosis cases in the local hospitals. The six markets from different districts differed in the level of contamination and strain types. Persistent contamination of L. monocytogenes was found in the markets especially in meat mincers, which were found to be one likely source of continuous cross contamination. These findings will help develop strategies to reduce L. monocytogenes contamination in open markets for better public health control and prevention of foodborne L. monocytogenes infections. |
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| Keywords: | Serotype MLST PFGE Persistence Cross contamination |
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