A novel developed method based on single primer isothermal amplification for rapid detection of Alicyclobacillus acidoterrestris in apple juice |
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| Affiliation: | 1. College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China;2. College of Science and Technology, Hebei Agricultural University, Cangzhou 061100, China;1. Polytechnic Department of Engineering and Architecture, via del Cotonificio 108, University of Udine, 33100 Udine, Italy;2. Department of Agricultural, Food, Environmental and Animal Sciences, via Sondrio 2/a, University of Udine, 33100 Udine, Italy;1. Department of Immunopathology of Infectious and Parasitic Diseases, Warsaw Medical University, 3C Pawinskiego Street, 02-106 Warsaw, Poland;2. Postgraduate School of Molecular Medicine, 61 Żwirki i Wigury Street, 02-091 Warsaw, Poland;3. Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University 3-1 Yamadaoka, Suita-City, Osaka, Japan;4. Department of Clinical Science, University of Bergen, 5021 Bergen, Norway;5. Municipal Hospital for Infectious Diseases, 37 Wolska Street, 01-201 Warsaw, Poland;6. Department of Genetics, Institute of Physiology and Pathology of Hearing, Mochnackiego 10, 02-042 Warsaw, Poland;7. Department of Neurology, Military Institute of Medicine, 128 Szaserów Street, 04-141 Warsaw, Poland |
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| Abstract: | Alicyclobacillus acidoterrestris (A. acidoterrestris), as one of the most major spoilage-causing species within the Alicyclobacillus genus, can survive and multiply in the pasteurization process. Nowadays, A. acidoterrestris has become worldwide issue in the fruit juice industry due to its spore-forming and thermo-acidophilic features. A novel single primer isothermal amplification assay (SPIA) was developed for the rapid detection of A. acidoterrestris in apple juice. This assay was designed to target the 16S rRNA gene with a DNA/RNA chimeric primer. Detection of gene amplification was accomplished by amplification curve, turbidity and addition of single strand DNA binding dye SYBR Green II allowing visualization under ultra violet light. The specificity of the assay was confirmed using 7 strains of A. acidoterrestris and 30 strains of non-A. acidoterrestris. The SPIA was highly sensitive and the detection limit was as low as 4.8 CFU/mL. Furthermore, the apple juice inoculated with 61 CFU/mL of A. acidoterrestris could be detected as positive. The novel SPIA with visualization results was successfully applied for the detection of A. acidoterrestris and exhibited high specificity and sensitivity, making it a powerful tool for the detection of A. acidoterrestris in fruit juice industry and being conveniently applied in developing countries with limited resource. |
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| Keywords: | Single primer isothermal amplification (SPIA) DNA/RNA chimeric primer Visualization Apple juice |
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