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Development of highly soluble and functional buffalo milk protein concentrate 60 by modifying ionic environment and characterisation thereof
Authors:Kuldeep Dudi  Sunil Kumar Khatkar
Affiliation:1. By-products Utilization Lab, Department of Dairy Technology, College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India

Contribution: Data curation, ​Investigation, Methodology, Writing - original draft;2. By-products Utilization Lab, Department of Dairy Technology, College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India

Abstract:The ionic and protein environment of buffalo skim milk was modified by instant pH drop and its restoration for the development of highly soluble milk protein concentrate-60 (BuMRate–60@), which resulted in depletion of 88.64% calcium, 89.18% magnesium, 91.69% potassium and 93.96% phosphorous. BuMRate–60@ displayed excellent wetting, rehydration and solubility (97.76%). The Fourier transform infrared spectrometer spectra revealed significant stretching (C-H and O-H), N-H bending and a large extent of H-bonding. Scanning electron microscopy micrographs presented particles with porous nature and dimples, while transmission electron microscopy confirmed a greater release of minerals with altered casein micelles.
Keywords:Buffalo milk  Casein  Ultrafiltration  Milk proteins  Acidification  Milk powders
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