Somatic cell recovery by microfiltration technologies: A novel strategy to study the actual impact of somatic cells on cheese matrix |
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| Affiliation: | 1. UMR STLO, INRA, Agrocampus Ouest, 65 rue de Saint Brieuc, 35000 Rennes, France;2. Actalia Dairy Products, BP 50915, 35009 Rennes Cedex, France;3. UMR GABI, INRA, AgroParisTech, domaine de Vilvert, 78350 Jouy-en-Josas, France;4. Plateforme @BRIDGe, 78350 Jouy-en-Josas, France;3. From the Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, Architecture et Réactivité de l''ARN, UPR 9002, F-67000 Strasbourg, France,;4. Université de Strasbourg, CNRS, Institut de Biologie Moléculaire des Plantes, UPR 2357, F-67084 Strasbourg, France,;5. Université de Strasbourg, CNRS, Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, F-67404 Illkirch, France,;6. Synchrotron SOLEIL, l''Orme des Merisiers, F-91410 Saint Aubin, France;1. IJPB, UMR1318 Inra/AgroParisTech, Inra, Route de Saint-Cyr, 78026 Versailles Cedex, France;2. ICMPE, UMR7182, Equipe ESO, 2-8 rue Henri Dunant, 94320 Thiais, France;2. Université de Lyon, UMR 5023 LEHNA, F-69100, Villeurbanne, France;3. INRA, USC IGH 1369, ENTPE, F-69518, Vaulx en Velin, France;4. Centre d''Ecologie Fonctionnelle et Evolutive (UMR 5175), Ecole Pratique des Hautes Etudes, Biogéographie et Ecologie des Vertébrés, Campus CNRS, 1919 Route de Mende, 34 293 Montpellier Cedex 5, France;5. Institut des Sciences Analytiques, Département Service Central d''Analyse, UMR 5280, 5 rue de la Doua, F-69100, Villeurbanne, France;1. Irstea, Bordeaux, France;2. IMB, Bordeaux, France;1. UR1268 Biopolymères Interactions Assemblages, INRA, F-44316 Nantes, France;2. CNR-IOM-OGG, c/o Institut Laue-Langevin, 6 rue Jules Horowitz, BP 156, 38042 Grenoble Cedex 9, France;3. Institut Laue-Langevin, 6 rue Jules Horowitz, BP 156, 38042 Grenoble Cedex 9, France;4. Université Joseph Fourier UFR PhITEM, BP 53, 38041 Grenoble Cedex 9, France;5. Institut de Biologie Structurale, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France;1. Department of Urology, Stanford University School of Medicine, CA;2. Veterans Affairs Palo Alto Health Care System, CA;3. Department of Surgery, Stanford University School of Medicine, CA;4. David Geffen School of Medicine and Olive View-UCLA Medical Center, Sylmar, CA;5. Veterans Administration Greater Los Angeles Healthcare System, CA |
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| Abstract: | The actual impact of the somatic cells in the dairy technology is still ill-defined, because the increase in milk somatic cell count, usually correlated with mastitis factors, impairs the raw milk composition, through mainly unwanted proteolysis and lipolysis. This study used microfiltration technologies for recovering high quantity of somatic cells and for clarifying their role in cheese quality. Three series of Swiss-type cheeses were manufactured by adding 0 (control), 4 × 105 and 9 × 105 somatic cells mL?1. These cells were traced for the first time during the cheese making process by using adapted flow cytometry and real-time quantitative PCR. Proteolysis and lipolysis indices were measured throughout ripening time. Only a weak increase in lipolysis (+28%) and proteolysis (+8%) was observed in the highest somatic cell count cheese, despite 73% of the cells trapped within the cheeses. Our approach gives a new view of somatic cell role in cheese milk alteration. |
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