Microbiological assay with Bacillus licheniformis for the easy detection of quinolones in milk |
| |
| Affiliation: | 1. Cátedra de Biofísica, Departamento de Ciencias Básicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, R.P.L Kreder 2804, 3080 Esperanza, Argentina;2. Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, Camino de Vera, s/n, 46071 Valencia, Spain;1. Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy;2. Institute of Nanostructured Materials, Consiglio Nazionale delle Ricerche, c/o Department of Chemistry, ‘Sapienza’ University of Rome, Piazzale Aldo Moro 5, 00186 Roma, Italy;3. External Relations Office, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy;4. National Reference Centre for Bovine Milk Quality, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Via Bianchi 9, 25124 Brescia, Italy;1. Research Group of Analytical Chemistry and Life Sciences, Department of Analytical Chemistry, University of Granada, Campus of Fuentenueva, E-18071 Granada, Spain;2. Research Group of Bioanalysis, Department of Analytical Chemistry, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba 171, E-08921 Sta. Coloma de Gramenet, Barcelona, Spain |
| |
| Abstract: | This paper proposes a microbiological method in microtitre plates for the detection of residues of quinolones in milk. The method uses spores of Bacillus licheniformis in culture medium with a redox combination of indicators and gives a response time of 5.5 h. This method detects 92 μg L?1 of ciprofloxacin, 63 μg L?1 of danofloxacin, 109 μg L?1 of enrofloxacin, 101 μg L?1 of marbofloxacin and 109 μg L?1 of sarafloxacin in milk. Therefore, the assay is easy to develop and to use in laboratory, allowing analysis of large numbers of samples at low cost. Due to its good sensitivity to quinolones, this assay can be used as a complementary test of commercial microbiological methods and thereby improve food security. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
