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Screening of aflatoxin M1 occurrence in selected milk and dairy products in Terengganu,Malaysia
Affiliation:1. Commonwealth Scientific and Industrial Research Organisation, Food and Nutrition, 671 Sneydes Road, Private Bag 16, Werribee, VIC 3030, Australia;2. Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC 3220, Australia;1. Laboratory of Quality & Safety Risk Assessment for Dairy Products of Ministry of Agriculture (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China;2. Milk and Dairy Production Inspection Center of Ministry of Agriculture (Beijing), Beijing, 100193, PR China;3. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, PR China;1. Escuela de Tecnología de Alimentos, 11501-2060, Ciudad Universitaria Rodrigo Facio San José, Costa Rica;2. Centro Nacional de Ciencia y Tecnología de Alimentos (CITA), 11501-2060, Ciudad Universitaria Rodrigo Facio San José, Costa Rica;3. Centro de Investigación en Nutrición Animal (CINA), Universidad de Costa Rica, 11501-2060, Ciudad Universitaria Rodrigo Facio San José, Costa Rica;4. Escuela de Zootecnia, Universidad de Costa Rica, 11501-2060, Ciudad Universitaria Rodrigo Facio San José, Costa Rica;5. Centro de Investigación en Enfermedades Tropicales (CIET), Universidad de Costa Rica, 11501-2060, Ciudad Universitaria Rodrigo Facio San José, Costa Rica;6. Facultad de Microbiología, Universidad de Costa Rica, 11501-2060, Ciudad Universitaria Rodrigo Facio San José, Costa Rica;1. Laboratory of Toxicology, Department of Basic Health Sciences, State University of Maringa (UEM), Colombo Avenue 5790, 87020-900 Maringa, Brazil;2. Department of Statistics, UEM, Brazil;1. Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055, Portici, Napoli, Italy;2. Department of Pharmacy, University of Napoli Federico II, Napoli, Italy
Abstract:The study was conducted to screen the occurrence of aflatoxin M1 (AFM1) in 53 selected milk and dairy product samples (11 liquid milk, 12 powdered milk, 8 3-in-1 beverages, 6 condensed sweetened milk, 2 evaporated milk, 7 cultured milk drink, 5 yogurt and 2 cheese samples). These samples were purchased from selected markets in Terengganu, Malaysia in January 2014 based on a questionnaire survey among 212 respondents on the types and brands of milk and dairy products that were frequently consumed. Based on the responses, 53 milk and dairy products were purchased and the competitive enzyme-linked immune-absorbent assay (ELISA) method was used to determine the level of AFM1 in the samples. Of 53 samples, 19 samples were positive with AFM1 (35.8%) ranging from 3.5 to 100.5 ng/L. Although 4/53 (7.5%) of the tested samples had the contamination level greater than the European Commission (EC) limit (>50 ng/L), the contamination levels were still below the Malaysia Food Regulation 1985 limit (less than 500 ng/L). This study provided a pioneering data on the occurrence of AFM1 in milk and dairy products in Malaysia.
Keywords:Aflatoxin  Milk and dairy products  Terengganu  Malaysia
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