Effects of high corn oil diet on preneoplastic murine colons: Prostanoid production and lipid composition |
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Authors: | Nancy M Robblee Ranjana P Bird |
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Affiliation: | (1) Department of Foods and Nutrition, University of Manitoba, Human Ecology Building, R3T 2N2 Winnipeg, Manitoba, Canada |
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Abstract: | In the present study, the effect of normal (5% by wt) and high (23.5% by wt) corn oil diets on prostanoid production and on
the lipid composition of preneoplastic colonic epithelium was investigated. CF1 mice (female, 3–4-weeks-old) were fed a normal corn oil dietad libitum and were treated with the colon carcinogen 1,2-dimethylhydrazine (DMH, 20 mg/kg/wk) or saline (control) for 24 wk. At this
stage, all animals received the AIN-76 diet (normal corn oil)ad libitum. Following the last injection, half of the animals from each treatment group were randomly allocated to a high corn oil diet
for 5 to 10 wk, whereas the remaining animals continued on the normal corn oil diet. After 5 wk of feeding, the colonic mucosa
of carcinogentreated animals had a higher level of bicyclic prostaglandin E2 (PGE2) than had the animals in the control groups; prostanoid synthesis in the colonic mucosa of control animals was unaffected
by the high corn oil diet. Preneoplastic colonic mucosa of animals fed the high corn oil diet had a significantly higher level
of PGE2 than corresponding control colonic mucosa. The 6-keto-prostaglandin F1α/thromboxane B2 ratio was significantly lower in the DMH-treated groups than in the control groups, and was unaffected by dietary treatments.
After 10 wk of feeding a particular diet, the differences in the fatty acid composition between the control and DMH-treated
groups were minor. Our findings demonstrate that the preneoplastic colonic epithelium differs from that of normal epithelium
with respect to prostanoid synthesis. |
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