Growth characteristics,biohydrogen production and photochemical activity of photosystems in green microalgae Parachlorella kessleri exposed to nitrogen deprivation |
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| Affiliation: | 1. Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia;2. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, 27 Akademicheskaya Str., 220072, Minsk, Belarus;3. Department of Plant Cell Biology and Bioengineering, Biology Faculty, Belarusian State University, 4 Independence Ave., 220030, Minsk, Belarus;4. International Research Centre for Environmental Membrane Biology, Foshan University, Foshan, 528041, China |
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| Abstract: | The biohydrogen (H2) generation in green microalgae is associated with electron transfer during photosynthesis. In this study, the ability of Parachlorella kessleri RA-002 isolated in Armenia to generate biohydrogen (H2) in TAP and Tamiya media with and without nitrogen deprivation have been determined. Nitrogen deprivation led to a suppression of the growth rate and a decrease in the content of photosynthetic pigments in algae, as well as to increase of H2 yield. The highest H2 yield was found during the growth of algae in TAP and Tamiya media under nitrogen-deprived conditions, which was 4–5 times more compared to control cells grown without nitrogen deprivation. Diuron inhibited this process, indicating that H2 generation was due to PS II activities. The analysis of the effect of nitrogen-deprived conditions on the activity of photosystem I and II has been carried out. Nitrogen deprivation suppressed of photosynthetic activity of PS II (up to 20% compared to control) that facilitated the formation of anaerobic conditions increasing the total yield of H2. |
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| Keywords: | Parachlorella kessleri Nitrogen deprivation Photosystems Photochemical activity |
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