Towards Lipidomics of Low-Abundant Species for Exploring Tumor Heterogeneity Guided by High-Resolution Mass Spectrometry Imaging |
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| Authors: | Jonathan Cimino David Calligaris Johann Far Delphine Debois Silvia Blacher Nor Eddine Sounni Agnès Noel Edwin De Pauw |
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| Affiliation: | 1.Mass Spectrometry Laboratory, GIGA-R, Department of Chemistry, University of Liege, Liege 4000, Belgium; E-Mails: (J.C.); (J.F.); (D.D.);2.Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege, Liege 4000, Belgium; E-Mails: (S.B.); (N.E.S.); (A.N.);3.Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA |
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| Abstract: | Many studies have evidenced the main role of lipids in physiological and also pathological processes such as cancer, diabetes or neurodegenerative diseases. The identification and the in situ localization of specific low-abundant lipid species involved in cancer biology are still challenging for both fundamental studies and lipid marker discovery. In this paper, we report the identification and the localization of specific isobaric minor phospholipids in human breast cancer xenografts by FTICR MALDI imaging supported by histochemistry. These potential candidates can be further confirmed by liquid chromatography coupled with electrospray mass spectrometry (LC-ESI-MS) after extraction from the region of interest defined by MALDI imaging. Finally, this study highlights the importance of characterizing the heterogeneous distribution of low-abundant lipid species, relevant in complex histological samples for biological purposes. |
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| Keywords: | MALDI mass spectrometry imaging FTICR mass spectrometry lipidomics hypoxia necrosis cancer |
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