Phospholipid profiling of sediments using phosphorus-31 nuclear magnetic resonance |
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Authors: | Lidia G. Bardygula-Nonn Jerry L. Kaster Thomas Glonek |
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Affiliation: | (1) Center for Great Lakes Studies, University of Wisconsin-Milwaukee, 53204 Milwaukee, Wisconsin;(2) MR Laboratory, Midwestern University, 5200 South Ellis Ave, 60615 Chicago, Illinois |
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Abstract: | A phosphorus-31 nuclear magnetic resonance method has been developed for the determination of aquatic sediment phospholipid profiles that may be generally applied to all soils and deposits containing viable cellular material. A method of scrubbing chloroform/methanol extracts with potassium acid phosphate overcomes adverse signal broadening from the mineral component, permitting eleven sediment phospholipids to be determined at the quantitative level. |
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