| Isolation of Leptospirillum ferriphilum by single-layered solid medium |
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| 引用本文： | 柳建设 谢学辉 肖升木 王秀美 赵文杰 田卓力. Isolation of Leptospirillum ferriphilum by single-layered solid medium[J]. 中南工业大学学报(英文版), 2007, 14(4): 467-473. DOI: 10.1007/s11771-007-0091-3 |
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| 作者姓名： | 柳建设 谢学辉 肖升木 王秀美 赵文杰 田卓力 |
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| 作者单位： | School of Mineral Processing and Bioengineering Central South University，School of Mineral Processing and Bioengineering Central South University，School of Mineral Processing and Bioengineering Central South University，School of Mineral Processing and Bioengineering Central South University，School of Mineral Processing and Bioengineering Central South University，School of Mineral Processing and Bioengineering Central South University，Changsha 410083 China College of Environmental Science and Engineering Donghua University Shanghai 201620 China，Changsha 410083 China，Changsha 410083 China，Changsha 410083 China，Changsha 410083 China，Changsha 410083 China |
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| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划) |
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| 摘 要： | According to physiological and biochemical characteristics of Leptospirillum ferriphilum,a strain of object bacteria was isolated successfully.Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium.Colony was cultured and bacteria were collected.The morphologies of the object bacteria were observed using crystal violet staining,scanning electron microscope(SEM)and transmission electron microscope(TEM).The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1.These results indicate that this new single-layered agar solid medium is efficient and simple for isolation of Leptospirillum ferriphilum.Additionally,physiological-biochemical characteristics show that the optimum initial pH value and its growth temperature are 1.68 and 40 ℃,respectively.The culture of it is used to leach a complex concentrate chalcopyrite,the leaching efficiencies of copper and iron are 1.93 % and 13.74 %,respectively,and it is more effective than the A.ferrooxidans culture in the leaching of the complex concentrate chalcopyrite.
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| 关 键 词： | 绝缘材料 固体介质 黄铜矿 微生物 |
| 收稿时间： | 2006-01-11 |
| 修稿时间： | 2006-01-112006-09-27 |
Isolation of Leptospirillum ferriphilum by single-layered solid medium |
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| Liu Jian-she , Xie Xue-hui , Xiao Sheng-mu , Wang Xiu-mei , Zhao Wen-jie and Tian Zhuo-li. Isolation of Leptospirillum ferriphilum by single-layered solid medium[J]. Journal of Central South University of Technology, 2007, 14(4): 467-473. DOI: 10.1007/s11771-007-0091-3 |
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| Authors: | Liu Jian-she Xie Xue-hui Xiao Sheng-mu Wang Xiu-mei Zhao Wen-jie Tian Zhuo-li |
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| Affiliation: | 1. School of Mineral Processing and Bioengineering, Central South University, Changsha 410083, China; 2. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China |
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| Abstract: | According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium. Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining, scanning electron microscope(SEM) and transmission electron microscope (TEM). The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and simple for isolation of Leptospirillum ferriphilum. Additionally, physiological-biochemical characteristics show that the optimum initial pH value and its growth temperature are 1.68 and 40 °C, respectively. The culture of it is used to leach a complex concentrate chalocpyrite, the leaching efficiencies of copper and iron are 1.93% and 13.74%, respectively, and it is more effective than the A. ferrooxidans culture in the leaching of the complex concentrate chalcopyrite. Foundation item: Projects (50374075, 50321402) supported by the National Natural Science Foundation of China; Project(2004CB619204) supported by the National Basic Research and Development Program of China; Project(200549) supported by the Specialized Research Fund of the National; Excellent PhD Thesis |
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| Keywords: | Leptospirillum ferriphilum isolation purification solid medium bioleaching concentrate chalcopyrite |
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