A SIMPLE SOLID-PHASE RADIOIMMUNOASSAY FOR AFLATOXIN B1 |
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Authors: | PIERA S SUN F S CHU |
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Affiliation: | Food Research Institute and Department of Food Microbiology and Toxicology The University of Wisconsin Madison, Wisconsin 53706 |
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Abstract: | A solid-phase radioimmunoassay (RIA) for aflatoxin B1 (afla B1) was developed. This method involved the incubation of afla B1, both labelled and unlabelled, with immunoglobulin (IgG)-sepharose gel which was prepared by conjugation of the IgG highly specific to afla B1 with CNBr-activated sepharose gel, followed by a filtration step. The binding capacity was determined by counting the radioactivity in the filtrate. Studies with different afla B1 analogues revealed that the IgG-gel bound most effectively with B1. Binding of afla B2, G1, G2, and aflatoxicol to the IgG-gel was less effective in comparison with the IgG before coupling. Between 0.5–5.0 ng per assay, the displacement of radioactivity from the gel was directly proportional to the amount of afla B1 present. Using a simple extraction procedure without clean-up step, the recovery yields for afla B1 in the contaminated corn or wheat at levels of 5 ppb or above were above 60%. |
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