Electrochemical study of S-nitrosoglutathione and nitric oxide by carbon fibre NO sensor and cyclic voltammetry - possible way of monitoring of nitric oxide |
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Authors: | Jan Vitecek Jiri Petrek David Potesil Ladislav Havel Libuse Trnkova |
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Affiliation: | a Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic b Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic c Department of Analytical Chemistry, Masaryk University Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic d Department of Theoretical and Physical Chemistry, Masaryk University Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic |
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Abstract: | Nitrosation of sulfhydryl group of glutathione, which is highly reactive and is often found conjugated to other molecules via its sulfhydryl moiety, is one of many biological effects of the nitric oxide (NO). This process may serve as a signal event and/or as a deposition of NO to S-nitrosoglutathione (GSNO). Moreover, GSNO may release NO under specific conditions. That is why NO, which has a little lifetime itself, could be distribute for longer distances within the organism. Here, we studied and compared the basic electrochemical characteristics of biological active thiol compounds (GSH, oxidized glutathione and GSNO). In addition, observation of the decomposition process of GSNO using different electrochemical techniques followed. Primarily we studied the influence of scan rate and reducing agent (Tris(2-carboxyethyl)phosphine). The CV calibration equations were linear, R.S.D. about 5%. The detection limits of GSH, GSSG and GSNO expressed as 3 S/N were 9 nM, 4 nM and 20 nM, respectively. In addition, the use of NO selective carbon fibre electrode and cyclic voltammetry for the study of GSNO decomposition catalysed by copper(II) and iron(II) followed. |
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Keywords: | Glutathione GSH GSSG Nitrosoglutathione S-nitroso-N-acety-D L-penicillamine Thiols Nitric oxide Cyclic voltammetry Carbon fibre electrode Copper Iron |
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