Semiconductor oxide based electrodes for the label-free electrical detection of DNA hybridization: Comparison between Sb doped SnO2 and CdIn2O4 |
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Authors: | V Stambouli A Zebda C Guiducci J-P Diard N Brack |
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Affiliation: | a LMGP, ENSPG, Domaine universitaire, BP 46, 38402 Saint-Martin d’Hères cedex, France b LEPMI, ENSEEG, Domaine universitaire, BP 75, 38402Saint Martin d’Hères cedex, France c Centre for Materials and Surface Science, Department of Physics, La Trobe University, Melbourne, Vic. 3086, Australia |
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Abstract: | First results are reported regarding the design, fabrication and operation of a DNA biochip based on a semiconductor oxide electrode that employs label-free electrical detection of the DNA hybridization. The same process of DNA functionalisation, including hydroxylation and silanization steps, was performed on two types of semiconductor oxide: Sb doped SnO2 and CdIn2O4 thin films. These oxide electrodes were laboratory-made films deposited on glass substrates using a chemical vapour deposition method, i.e. the aerosol pyrolysis technique. After having characterized some physico-chemical properties of the bare films, the label-free electrical DNA hybridization detection, without redox couple labelling, was performed using electrochemical impedance spectrometry (EIS) before and after hybridization. On both oxides, over a large frequency range, a significant increase in the impedance modulus was obtained. The increase in the case of CdIn2O4 was by a factor of 2.1 ± 0.5 and in the case of Sb doped SnO2 was by a factor of 1.6 ± 0.1. This phenomenon was especially marked on CdIn2O4 thin films, which exhibit a higher sensitivity to the surface event. The DNA hybridization to complementary DNA targets labelled with fluorescent markers was confirmed using fluorescence microscopy. |
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Keywords: | CdIn2O4 film Doped SnO2 film Label-free electrical detection DNA biochip |
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