Hot electron-induced cathodic electrochemiluminescence of rhodamine B at disposable oxide-coated aluminum electrodes |
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Authors: | Qinghong Jiang,Markus Hå kansson,Timo Ala-Kleme |
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Affiliation: | a Laboratory of Analytical and Inorganic Chemistry, Helsinki University of Technology, P.O. Box 6100, FIN-02015 HUT, Finland b Laboratory of Analytical Chemistry University of Turku, FIN-20014 Turku, Finland |
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Abstract: | Rhodamine B (RhB) exhibits strong cathodic electrogenerated chemiluminescence (ECL) in aqueous solutions during high-amplitude pulse polarization at thin oxide film-coated aluminum electrodes. This method allows the detection of RhB below nanomolar concentration level and provides linear calibration plots spanning over several orders of magnitude of concentration. In addition, a relatively long ECL lifetime of RhB provides a basis for time-resolved detection. Thus, widely used RhB-based labels can also be suggested to be usable as electrochemiluminescent labels in fully aqueous solutions in bioaffinity assays such as in immunoassays and DNA-probing assays. Support was obtained for the chemiluminescence generation mechanism to be essentially the same as that of radiochemiluminescence in aqueous solution. |
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Keywords: | Electrochemiluminescence (ECL) Time-resolved detection Rhodamine B Hot electron Tunnel emission Oxide-coated aluminum electrode |
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