基于表面增强拉曼光谱适配体传感器检测植物蛋白肉中的黄曲霉毒素B1

目的 随着生活水平的提高，经济社会的发展，面对丰富多样的食品种类，保证饮食安全已成为民众关注的重点。然而，食品中的黄曲霉毒素B1（Aflatoxin B1，AFB1）的存在对人类健康构成了极大的威胁。因此，开发一种快速、简单、灵敏的AFB1检测方法，对食品安全十分必要和迫切。方法 研发了一种检测AFB1的表面增强拉曼散射(surface enhancement of Raman scattering, SERS)适配体传感器，传感器由巯基适配体（SH-DNA）修饰的金包四氧化三铁磁性纳米粒子（Fe3O4@AuMNPs）作为捕获底物与巯基适配体互补链（SH-cDNA）修饰的金包二氧化硅纳米粒子（SiO2@Au-MBANPS）作为信号探针所组成。通过适配体与AFB1的特异性结合可以导致捕获底物释放信号探针，SERS信号强度随着AFB1的浓度的变化而发生变化，从而实现对AFB1的检测。结果 通过实验条件的优化，得到线性回归方程为Y= -222.07lgX+ 5723.12，r2= 0.9986的标准曲线，检出限为0.37 pg/mL。该SERS传感器成功应用于实际样品中AFB1的分析，得到回收率为98% ~ 110%，相对标准偏差值为3.9% ~ 5.7%。结论 本研究建立的SERS适配体传感系统可以实现对AFB1的定量检测，可以清楚识别AFB1与其他毒素的SERS信号强度差别，为AFB1的快速检测提供了新的检测方法，在实际样品植物蛋白肉中表现出优异的检测性能。

Detection of aflatoxin B1 in vegetable protein meat based on surface enhanced Raman spectral aptamer sensor

Objective With the improvement of living standards and the development of economy and society, ensuring food safety has become the focus of public concern in the face of rich and diverse food types. However, Aflatoxin B1 (AFB1) in food poses a great threat to human health. Therefore, it is necessary and urgent to develop a fast, simple and sensitive AFB1 detection method for food safety. Methods Surface enhancement of Raman scattering (SERS) aptamer sensor for AFB1 detection was developed. The sensor was composed of a sulfhydryl aptamer (SH-DNA) modified ferro-tetroxide magnetic nanoparticle (Fe3O4@Au) as a trapping substrate and a sulfhydryl aptamer complementary chain (SH-cDNA) modified ferro-tetroxide magnetic nanoparticle (SiO2@Au-MBA) as a signal probe. The specific binding of aptamer and AFB1 can trigger the captured substrate release signal probe, resulting in the SERS signal intensity changing with the concentration of AFB1, so as to realize the detection of AFB1. Results By optimizing the experimental conditions, the standard curve with linear regression equation Y= -222.07lgX+ 5723.12, r2= 0.9986 was obtained, and the detection limit was 0.37 pg /mL. The SERS sensor was successfully applied to the analysis of AFB1 in actual samples, and the recovery rate was 98% ~ 110%, and the relative standard deviation was 3.9% ~ 5.7%. Conclusion The SERS aptamer sensing system established in this study could realize the quantitative detection of AFB1 and clearly identify the difference in SERS signal strength between AFB1 and other toxins, providing a new detection method for the rapid detection of AFB1 and showing excellent detection performance in the actual sample plant protein meat.