Influence of formate on bioactivity material-thuringiensin synthesized by <Emphasis Type="Italic">Bacillus thuringiensis</Emphasis> YBT-032 |
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Authors: | Zhi Wang Xiong Chen Shouwen Chen Ming Sun Ziniu Yu |
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Affiliation: | (1) College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China;(2) State Key Laboratoy of Agricultural Microbiology National Engineering Research Center for Microbial Posticides, Huazhong Agricultural University, Wuhan, 430070, China |
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Abstract: | The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated.
Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α-ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of
cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source,
formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues,
and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g−1·h−1 and transformation ratio from glucose to thuringiensin increased by 43.30%.
Funded by the National Natural Science Foundation of China (No.30170032) Affiliated institutions 1 and 2 contributed equally
to this work |
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Keywords: | bacillus thuringiensis precursor formate thuringiensin |
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