Effects of highly purified sardine proteins on lipid peroxidation and reverse cholesterol transport in rats fed a cholesterol-rich diet |
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Authors: | Sabrine Louala Sherazed Hamza-Reguig Aicha Benyahia-Mostefaoui Ahmed Boualga Myriem Y Lamri-Senhadji |
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Affiliation: | Laboratoire de Nutrition Clinique et Métabolique (LNCM), Faculté des Sciences, Département de Biologie, Université d’Oran, 31000 Oran, Algeria |
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Abstract: | The effect of highly purified sardine proteins was compared with that of casein on serum and lipoproteins lipid peroxidation and reverse cholesterol transport. Lecithin:cholesterol acyltransferase (LCAT) activity, high density lipoproteins (HDL2 and HDL3) composition and serum lipid and lipoproteins peroxidation were determined in rats fed a cholesterol-rich diet. Hypercholesterolemic rats were divided into two groups fed diets enriched with cholesterol and containing 20% of highly purified sardine proteins (SPc) or casein (CASc) for 28 days. A control group was fed a standard diet (CAS). Compared with CAS and CASc, the thiobarbituric acid reactive substances (TBARS) concentrations of low density lipoprotein (LDL)–HDL1 in SPc were 3.5- and 1.7-fold higher compared with casein diets. TBARS in HDL2 and HDL3 were, respectively, 2.3- and 1.6-fold lower in SPc compared with CASc. In SPc group, LCAT activity was higher compared to CASc and CAS (P < 0.05). Purified sardine proteins had no beneficial effects on LDL-cholesterol and lipid peroxidation. However, they reduced HDL oxidation and improved reverse cholesterol transport, in the hypercholesterolemic rat. |
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Keywords: | Abbreviations: CAS rats fed casein diet CASc hypercholesterolemic rats fed casein diet containing cholesterol HDL high density lipoprotein LCAT lecithin:cholesterol acyltransferase LDL low density lipoprotein MUFA monounsaturated fatty acid SPc hypercholesterolemic rats fed sardine protein diet containing cholesterol PUFA polyunsaturated fatty acid SFA saturated fatty acid TBARS thiobarbituric acid reactive substance TC total cholesterol TG triacylglycerol |
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