β-半乳糖苷酶基因(lacZ)在海藻裙带菜中的稳定表达

借鉴海带遗传转化模式，利用基因枪法将β-半乳糖苷酶基因(lacZ)导入裙带菜雄配子体中，一部分诱导形成营养增殖的丝状体，另一部分和雌配子体受精生成孢子体。五个月后组织化学染色结果表明5％的孢子体个体和30％的丝状体细胞表现了lacZ的稳定表达，利用PCR和PCR-Southem检测得到了预期的阳性信号。本文结果提示了利用基因枪转化方法．以雄配子体介导获得外源基因在裙带菜中稳定表达的可行性．

Stable Expression of lacZ Reporter Gene in Seaweed Undaria pinnatifida

By using microprojectile bombardment method, lacZ reporter gene was transformed into male gametophytes of Undaria pinnatifida, which were then inbred with female gametophytes to generate sporophytes. After about five months, lacZ expression was detected in about 5% of regenerated sporophytes and 30% of unfertilized male gametophytes by histochemical staining. Results of PCR and PCR Southern analyses showed expected positive signals. The results suggested that male gametophytes could be applicable recipient of foreign genes to get stable expression.