| 人工合成的单链甜蛋白monellin基因在大肠杆菌中的高效表达 |
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| 引用本文： | 陈忠军,路福平,蔡恒,杜连祥.人工合成的单链甜蛋白monellin基因在大肠杆菌中的高效表达[J].食品与发酵工业,2005,31(9):18-20. |
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| 作者姓名： | 陈忠军 路福平 蔡恒 杜连祥 |
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| 作者单位： | 天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300222 |
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| 摘 要： | 根据已报道的单链monellin甜蛋白的氨基酸序列,采用细菌偏爱密码子,人工合成了全长294bp的monellin基因。插入到大肠杆菌表达载体pET22b中,构建重组分泌型表达载体pETMO。经IPTG诱导pETMO所含有的甜蛋白基因可在大肠杆菌BL21(DE3)中高效表达,表达量占菌体可溶性蛋白的44.8%。且经纯化后测定其甜度是蔗糖的3000倍。
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| 关 键 词： | 甜蛋白monellin 细菌优化密码子 重组PCR 诱导表达 |
| 收稿时间： | 03 23 2005 12:00AM |
| 修稿时间： | 07 4 2005 12:00AM |
High-level Expression of A Sweet Protein, Single-chain Monellin, in E. coli |
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| Chen Zhongjun,Lu Fuping,Cai Heng,Du Lianxiang.High-level Expression of A Sweet Protein, Single-chain Monellin, in E. coli[J].Food and Fermentation Industries,2005,31(9):18-20. |
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| Authors: | Chen Zhongjun Lu Fuping Cai Heng Du Lianxiang |
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| Abstract: | According to the amino acid sequence of monellin, a single chain 294bp monellin gene was synthesized based on E.coli biased codons. The fragment was inserted into vector pET-22b to construct the recombinant secretary plasmid pETMO. Induced by IPTG, monellin could be produced at a high level in E.coli BL21 (DE3), accounts for 44.8% of the soluble protein. The E.coli expressed single-chain monellin was 3000 times sweeter than sucrose. |
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| Keywords: | monellin bacteria preferred condon recombination PCR inducing expression |
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