TribUTE-F assay: Fluorescence-based rapid quantification of dietary intake in the red flour beetle,Tribolium castaneum (Coleoptera: Tenebrionidae), facilitates evaluation of antifeedant inhibitory effects |
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| Affiliation: | 1. Institute of Pesticides and Environmental Protection, Banatska 31b, POB 163, 11080, Belgrade-Zemun, Serbia;2. Institute for Medicinal Plants Research “dr Josif Pančić”, Tadeuša Košćuška 1, 11000, Belgrade, Serbia;3. University of Belgrade, Faculty of Agriculture, Institute for Phytomedicine, 11080, Belgrade, Serbia;1. Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guiyang University, Guiyang 550005, China;2. College of Tobacco Science, Guizhou University, Guiyang 550025, China;3. College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, China;1. Department of Agriculture and Fisheries, Queensland, Ecosciences Precinct, GPO Box 267, Brisbane, QLD, 4001, Australia;2. Plant Biosecurity Cooperative Research Centre, GPO Box 5012, Bruce, ACT, 2617, Australia;1. School of Biological Sciences, The University of Queensland, Brisbane, Queensland, 4072, Australia;2. Plant Biosecurity Cooperative Research Centre, LPO Box 5012, Bruce, ACT, 2617, Australia;3. Department of Agriculture and Fisheries, Queensland, Ecosciences Precinct, GPO Box 267, Brisbane, Queensland, 4001, Australia;4. Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA, 6151, Australia;5. New South Wales Department of Primary Industries, Private Mail Bag, Wagga Wagga, NSW, 2650, Australia |
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| Abstract: | Tribolium castaneum (Herbst.) is an important pest of stored grains, nuts, and cereals worldwide. Different methods have been used to estimate dietary intake and feeding preferences of this beetle. A previously described TribUTE (Tribolium Urges To Eat) assay estimates its dietary intake by measuring the amount of gypsum excreta in beetles fed gypsum, a non-digestible and non-toxic compound, using an analytical microbalance. The method revealed the sweet preferences of T. castaneum adults. However, the measurements based on microbalance require individual quantitation of each sample, and the weight of the samples is sometimes below the detection limit of the microbalance. Here, an improved TribUTE assay, designated TribUTE-F assay, that uses gypsum labeled with the fluorescence dye ROX as an endogenous tracer is described. The fluorescence intensity remains constant during the consumption and excretion of the labeled gypsum and it is strongly correlated with the amount of gypsum. This approach was then used to evaluate the inhibitory effect of an antifeedant, mulberry latex, on dietary intake of T. castaneum adults. Compared with microbalance measurements, the new method enables more accurate measurement of near the minimum detectable quantities and is a suitable tool for the discovery of antifeedants. |
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| Keywords: | Fluorescence Dietary intake High-throughput Calcium sulfate dihydrate Antifeedant ROX" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" 5(6)-Carboxy-X-rhodamine |
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