Alpha-amylase inhibitor from local common bean selection: Effect on growth and development of Corcyra cephalonica |
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| Affiliation: | 1. School of Biological Sciences, The University of Queensland, Brisbane, Queensland, 4072, Australia;2. Plant Biosecurity Cooperative Research Centre, LPO Box 5012, Bruce, ACT, 2617, Australia;3. Department of Agriculture and Fisheries, Queensland, Ecosciences Precinct, GPO Box 267, Brisbane, Queensland, 4001, Australia;4. Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA, 6151, Australia;5. New South Wales Department of Primary Industries, Private Mail Bag, Wagga Wagga, NSW, 2650, Australia;1. University of Catania, Dipartimento di Agricoltura, Alimentazione e Ambiente, Via Santa Sofia, 100, 95123, Catania, Italy;2. Humboldt-University of Berlin, Faculty of Life Sciences, Lentzeallee 55/57, 14195, Berlin, Germany;3. University Hohenheim, Institut für Zoologie, Fachgebiet Tierökologie 220c, Garbenstrasse 30, 70593, Stuttgart, Germany;1. Institut de Recherche pour le Développement (IRD), UMR 224-CNRS 5290-Université de Montpellier 1-Université de Montpellier 2 MIVEGEC (Maladies Infectieuses et Vecteurs: Ecologie, Genetique, Evolution et Contrôle), team BEES (Biology, Ecology and Evolution of vector Systems), 911 Avenue Agropolis, BP 64501, 34394 Montpellier, France;2. Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l’Ouest (DRO), 399 Avenue de la Liberté, 01 BP 545 Bobo Dioulasso, Burkina Faso |
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| Abstract: | Cultivars of common bean (Phaseolus vulgaris L.) from the Kalpa valley of the Kinnaur district were screened for alpha-amylase inhibitor activity against porcine pancreatic α-amylase and significant differences were found amongst them. The inhibitor protein was partially purified from Triloki cultivar by ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography. Inhibitor protein was non-competitive, heat labile and retained 55.51 per cent activity at 70 °C. It had two pH optima of 5.0 and 7.6. Inhibitor protein inhibited amylases of 3rd -4th instar larval extracts of Corcyra cephalonica Stainton, the rice moth. C. cephalonica larvae (3rd -4th instar) were fed on wheat flour mixed with 0.013 percent (w/w) of partially purified α-amylase inhibitor protein. The growth of larvae in the treatment was significantly reduced. Treated larvae showed 100 percent mortality after 11 days. In the untreated control, no larval mortality was observed and adults emerged after 45 days. The inhibitor protein was effective at very low concentrations and could be exploited for control of this grain storage pest. |
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| Keywords: | α- amylase inhibitor |
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