Sensitivity models in simulation of capillary zone electrophoresis: the effect of buffer power and initial buffer concentration

Separation of proteins by capillary zone electrophoresis in an amphoteric medium is investigated by numerical simulation. We explain how sensitivity models can be used to gain further information about this process. The sensitivity models that are derived are used to study the sensitivity of the separation to the specific buffer capacity of the amphoteric molecule that serves as a buffer. The effect of changing the overall concentration of this ampholyte is also examined and it is shown that a change in its concentration is equivalent to a change in its buffer capacity.