| 离子注入与紫外诱变筛选产高级醇低的果酒酵母 |
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| 引用本文： | 赵树欣,李春明. 离子注入与紫外诱变筛选产高级醇低的果酒酵母[J]. 酿酒科技, 2006, 0(11): 31-34 |
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| 作者姓名： | 赵树欣 李春明 |
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| 作者单位： | 天津工业微生物重点实验室,天津科技大学生物工程学院,天津,300222;天津工业微生物重点实验室,天津科技大学生物工程学院,天津,300222 |
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| 摘 要： | 采用离子注入法对一株自黎族酒曲中分离得的酵母TQ601诱变选育，通过乳酸培养基、产酸培养基和TTC上层平板初筛，再经果汁发酵复筛，并用气相色谱法测定果酒中高级醇含量，得到16株菌，其中TQ105菌的高级醇产量降低了34．5％。为防止发生回复突变，选择TQ111菌对其进行紫外诱变以巩固诱变效果。最终得到7株菌高级醇的产量均低于原始菌种TQ601，而且遗传稳定性较好。
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| 关 键 词： | 微生物 果酒酵母 离子注入 紫外诱变 高级醇 |
| 文章编号： | 1001-9286(2006)11-0031-04 |
| 收稿时间： | 2006-07-17 |
| 修稿时间： | 2006-07-17 |
Screening of Fruit Wine Yeast With Low Higher Alcohol Yield by Ion Implantation and UV Mutagenesis |
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| ZHAO Shu-xin,LI Chun-ming. Screening of Fruit Wine Yeast With Low Higher Alcohol Yield by Ion Implantation and UV Mutagenesis[J]. Liquor-making Science & Technology, 2006, 0(11): 31-34 |
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| Authors: | ZHAO Shu-xin LI Chun-ming |
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| Affiliation: | Tianj in of Industrial Microbiology Key Lab,College of Bioengineering, Tianjin University of Science and Technology, Tianj in 300222 |
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| Abstract: | By ion implantation of an original strain TQ601(separated from starter of Ni Minority) and then petri-dish screening on lactic acid,YEPD-CaCO3 and TTC and then secondary screening through fruit juice fermentation,16 strains with low higher alcohol yield were eventually obtained.The higher alcohol yield of all the 16 strains was measured by gas chromatography.Higher alcohol yield of TQ105 strain decreased by 34.5 %.To prevent resorting mutation,TQ111 strain was selected for UV mutagenesis and 7 strains with low higher alcohol yield and satisfactory heredity stability(their higher alcohol yield lower than original strain TQ601) were finally obtained. |
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| Keywords: | microbe fruit wine yeast ion implantation UV mutagenesis higher alcohols |
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