| 市售鸡蛋蛋白质图谱分析与真伪调查 |
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| 引用本文： | 杨国武,林霖,赖心田,黄秀丽,李传礼. 市售鸡蛋蛋白质图谱分析与真伪调查[J]. 食品工业科技, 2010, 0(1) |
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| 作者姓名： | 杨国武 林霖 赖心田 黄秀丽 李传礼 |
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| 作者单位： | 1. 深圳市计量质量检测研究院,广东深圳,518055
2. 深圳市计量质量检测研究院,广东深圳,518055;中山大学生命科学院,广东广州,510275 |
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| 摘 要： | 目的:分析市售鸡蛋的蛋白图谱,找出共性,建立鸡蛋稳定的蛋白质模式图谱,作为真假鸡蛋鉴别的检测方法。方法:对鸡蛋蛋黄的蛋白质进行等电聚焦(pH4～7)、SDS-PAGE电泳,银染后扫描,对获得的双向图谱用软件进行分析。结果:根据18个市售鸡蛋与真鸡蛋的蛋黄蛋白质图谱对比,获得三个共有的高丰度点群(共17个蛋白质点),分子量分别为97.2～66.4、66.4～44.3、44.3～29.0kDa,可作为鉴别真假鸡蛋的蛋白质模式图谱。市售鸡蛋蛋黄的蛋白质图谱与真鸡蛋相似,没有发现异样情况,没有在市场上发现假鸡蛋。结论:初步建立了鉴别真假鸡蛋的蛋白质组学方法,所建立的检测方法具有高度的重复性和准确性。
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| 关 键 词： | 鸡蛋 真伪 检测 蛋白质组学 |
Analysis of the proteins two-dimensional electrophoresis maps and research on authenticity of eggs sold in the markets |
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| YANG Guowu,LIN Lin,LAI Xin-tian,HUANG Xiu-li,LI Chuan-li. Analysis of the proteins two-dimensional electrophoresis maps and research on authenticity of eggs sold in the markets[J]. Science and Technology of Food Industry, 2010, 0(1) |
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| Authors: | YANG Guowu LIN Lin LAI Xin-tian HUANG Xiu-li LI Chuan-li |
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| Affiliation: | YANG Guo-wu1,LIN Lin1,2,LAI Xin-tian1,HUANG Xiu-li1,LI Chuan-li1(1. Shenzhen Academy of Metrology , Quality Inspection,Shenzhen 518055,China,2. School of Life Sciences,Zhongshan University,Guangzhou 510275,China) |
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| Abstract: | Objective:To establish a detection method for distinguishing real eggs from man-made eggs by finding the common model proteins map through two-dimensional electrophoresis.Method:Acquired the egg yolk's proteins maps by isoelectric focusing,SDS-PAGE and silver stain.The maps then had been scanned into computer and analyzed by software.Result:Compared proteins maps of 18 market-sold egg samples to that of the two real hen eggs,showed that there were three mutual abundant protein spots groups(17 protein spots ... |
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| Keywords: | egg authenticity detection proteomics |
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