基于贻贝仿生化学改善聚己内酯纤维多孔支架的 细胞相容性

在组织工程领域,支架的表面化学性能对调控细胞的生长行为起着关键的作用。为进一步改善聚己内酯(PCL)纤维支架的细胞相容性,开发了一种基于贻贝仿生化学在PCL纤维支架表面接枝生物相容性大分子的方法。该方法主要包含多巴胺在PCL纤维的表面涂覆和自聚合,以及生物活性大分子精氨酸-甘氨酸-天冬氨酸(RGD)和肝素的引入。傅里叶变换红外光谱测试结果表明RGD和PDA被成功地引入到PCL纤维支架表面。扫描电镜形貌检测和水接触角测试结果表明该改性手段不仅增大了纤维支架的表面粗糙度并且改善了支架的表面润湿性能。血管内皮细胞在改性的支架表面表现出了良好的细胞黏附性和细胞存活性。这种不涉及任何有毒溶剂的改性方法在组织工程支架领域具有广阔的应用前景。

Enhancing the Biocompatibility of PCL Nanofibrous Scaffolds Based on Mussel-Inspired Modification

The surface chemistry of the scaffolds plays vital role in regulating cell behavior in the tissue engineering. In order to enhance the biocompatibility of the polycaprolactone (PCL) scaffolds, a mussel-inspired modification and multiple bioactive molecules grafting method was developed. The modification method contained coating and polymerization of dopamine, the immobilization of bioactive molecules arginine-glycine-aspartic acid (RGD), and heparin. Fourier transform infrared spectroscopy (FTIR) results verified the successful polydopamine (PDA) coating and heparin grafting on the PCL scaffolds. Scanning electron microscope (SEM) and water contact angle tests verified the modification not only increased the nanofiber surface roughness, but also efficiently enhanced their surface wettability. Moreover, human umbilical vein endothelial cells (HUVECs) showed enhanced cell attachment, higher cell viability and greater cell proliferation on the RGD immobilized PCL electrospun nanofibers, which indicated great application prospect of this green method in the tissue engineering fields.