Use of PCR for Detecting Aspergillus flavus in Maize Treated by Gamma Radiation Process |
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| Authors: | Simone Aquino Ralf Greiner Ursula Konietzny Regina H Hassegawa Tatiana Alves dos Reis Benedito Corrêa |
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| Affiliation: | 1. Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP , Brazil siaq06@hotmail.com;3. Federal Research Center for Nutrition and Food , Center for Molecular Biology , Karlsruhe, Germany;4. Instituto de Ciências Biomédicas, Departamento de Microbiologia , USP, Brazil |
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| Abstract: | The aim of this study was to verify the effects of gamma radiation process on the fungal DNA and the application of PCR in the detection of Aspergillus flavus in irradiated maize grains. The samples were inoculated with a toxigenic strain and incubated under controlled conditions of relative humidity, water activity, and temperature for 15 days. After incubation, the samples were treated with gamma radiation with doses of 5 and 10 kGy and individually analyzed. The use of PCR technique showed the presence of DNA bands of Aspergillus flavus in all irradiated samples that showed no fungal growth in agar medium. |
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| Keywords: | gamma radiation Aspergillus flavus aflatoxin maize PCR DNA |
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