| 高左旋多巴合成活性的重组大肠杆菌培养条件优化 |
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| 引用本文： | 李华钟 孙伟 陈坚. 高左旋多巴合成活性的重组大肠杆菌培养条件优化[J]. 食品与生物技术学报, 2001, 20(3): 265-269 |
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| 作者姓名： | 李华钟 孙伟 陈坚 |
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| 作者单位： | 无锡轻工大学生物工程学院, |
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| 摘 要： | 对重组大肠杆菌进行培养条件的研究，得到细胞合成L-DOPA最佳培养条件为L-tyrosine0.2%、KH
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| 关 键 词： | 高左旋多巴；大肠杆菌 优化 |
| 文章编号： | 1009-038X(2001)03-0265-05 |
| 修稿时间： | 2000-10-16 |
Optimization of CultureConditions for Recombinant E.coliContaining High Activity of L-DOPA Synthesis |
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| LI Hua zhong,SUN Wei,CHEN Jian. Optimization of CultureConditions for Recombinant E.coliContaining High Activity of L-DOPA Synthesis[J]. Journal of Food Science and Biotechnology, 2001, 20(3): 265-269 |
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| Authors: | LI Hua zhong SUN Wei CHEN Jian |
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| Abstract: | Culture conditions for recombinant E.coli containing high tyrosine phenol lyase were studied. Optimum condition of enzyme formation was cells cultured at 37 ℃for 16hours in the medium containing 0.2% L-tyrosine, 0.2%KH2PO4, 0.1% MgSO4*7H2O, 0.001% FeSO4*7H2O, 0.001% ZnSO4*7H2O, 0.005% pyridoxal phosphate, 0.5% meat extract, 2% polypeptone, and 0.1% DL-Alanine. Initial pH and rotation rate were 7.0 and 150 r/min respectively. |
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| Keywords: | high L DOPA E.coli optimization |
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