| 五味子废渣中多糖的分离及体外抗氧化活性 |
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| 引用本文： | 汪艳群,孟宪军,刘丽,李斌,张琦,孙希云.五味子废渣中多糖的分离及体外抗氧化活性[J].食品与生物技术学报,2013,32(2):163-168. |
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| 作者姓名： | 汪艳群 孟宪军 刘丽 李斌 张琦 孙希云 |
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| 作者单位： | 沈阳农业大学食品学院,辽宁沈阳,110866 |
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| 基金项目： | 农业部公益性行业(农业)科技专项经费资助项目 |
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| 摘 要： | 为了充分利用提取木脂素后的五味子废渣,提高其附加值,采用水提醇沉法提取五味子粗多糖,经聚酰胺柱层析及Sephacryl S-300 HR凝胶柱层析对其进行纯化,将得到的级分SCP-0采用Sephacryl S-300 HR凝胶柱层析法及凝胶渗透色谱法进行纯度鉴定;然后对SCP-0进行体外清除自由基活性测定。结果显示,其对.OH具有较好的清除效果,IC50为14.5 mg/mL;对DPPH.具有较好的清除效果,IC50为2.1 mg/mL;但对.O2-无明显清除效果;具有一定的抗油脂氧化能力,在低自由基浓度水平下效果更明显。
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| 关 键 词： | 五味子 多糖 分离 自由基 抗氧化 |
Separation and Antioxidant Properties in Vitro of Polysaccharide from Schisandra chinensis Fruits Wastes |
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| WANG Yan-qun,MENG Xian-jun,LIU Li,LI Bin,ZHANG Qi and SUN Xi-yun.Separation and Antioxidant Properties in Vitro of Polysaccharide from Schisandra chinensis Fruits Wastes[J].Journal of Food Science and Biotechnology,2013,32(2):163-168. |
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| Authors: | WANG Yan-qun MENG Xian-jun LIU Li LI Bin ZHANG Qi and SUN Xi-yun |
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| Affiliation: | (College of Food Sciences,Shenyang Agricultural University,Shenyang 110866,China) |
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| Abstract: | The aim of this study is to find a way to reuse Schisandra chinensis fruits wastes after lignans extration.Polysaccharide tabled as SCP-0 was separated by hot-water extraction and ethanol precipitation,and then purified by polyamide column chromatography and Sephacryl S-300 HR column chromatography.After purity identification,the antioxidant activities of SCP-0 were investigated and the results showed a noticeable scavenging activity against hydroxyl radical and DPPH with IC50 of 14.5 mg/mL and 2.1 mg/mL respectively.However,superoxide anion scavenging activity of SCP-0 was hardly observed and certain ability of restraint on lard oxidation was observed. |
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