Dielectric behaviour of neutrophil gelatinase associated lipocalin detected by alpha relaxation process |
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| Authors: | Mukta Tyagi Amit Choudhary Santosh K Upadhyay Ved V Agrawal |
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| Affiliation: | 1. Academy of Scientific and Innovative Research (AcSIR);2. Biomedical Instrumentation Section, CSIR – National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi, India;3. Department of Physics, Deshbandhu College (University of Delhi), Kalkaji, New Delhi;4. CSIR-Institute of Genomics and Integrative Biology, New Delhi, India |
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| Abstract: | ABSTRACTWe have investigated alpha (α) relaxation process and its temperature evolution in electrical double layer (EDL) around neutrophil gelatinase-associated lipocalin (NGAL), anti-lipocalin2 (anti-LCN-2) and their conjugate in phosphate buffer. The dielectric permittivity (20 Hz to 1 MHz) of α-relaxation process for LCN-2 and anti-LCN-2 shows variation with temperature. This happens due to unfolding of various microstates of protein at measured temperature intervals. The DSC thermograms of LCN-2, anti-LCN-2 and their conjugate predict that the multiple unfolding domains are parallel to the shifts in real dielectric permittivity at a particular temperature. |
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| Keywords: | Alpha relaxation process lipocalin-2 acute kidney injury dielectric spectroscopy |
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