Proteomic approach for the detection of chicken mechanically recovered meat |
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Authors: | Surowiec I Koistinen K M Fraser P D Bramley P M |
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Affiliation: | School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK. |
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Abstract: | Mechanically recovered meat is cheaper than raw meat and thus has been incorporated into many meat-derived products. EU regulations exclude mechanically recovered meat from the definition of meat; as a consequence analytical procedures are needed to differentiate it from hand-deboned meat. The present pilot study has utilized a proteomic approach to find potential markers for the detection of chicken mechanically recovered meat. Intact proteins were extracted from raw meat and then analyzed with OFF-GEL electrophoresis followed by SDS-PAGE and identification of potential markers by nano-LC-MS/MS. It was shown that it is possible to extract, separate and identify key proteins from processed meat material. Potential chicken mechanically recovered meat markers--hemoglobin subunits and those similar to myosin-binding protein C were also identified. |
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Keywords: | Chicken meat analysis Mechanically recovered meat Hand deboned meat OFF-GEL electrophoresis Nano-LC-MS/MS Protein identification |
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