体外胃肠道消化及跨膜转运对干腌火腿肽抗炎活性的影响

以宣威火腿为原料提取火腿中生物活性肽，通过体外模拟胃肠道消化和Caco-2细胞单层膜转运实验，进一步分析火腿肽的序列及抗炎功能变化。结果表明：经模拟胃肠道消化后，宣威火腿肽的总体数量增加，其中1 000 Da以下多肽的数量占比从40.18%增加到57.40%；模拟消化后火腿肽抑制RAW264.7巨噬细胞炎症因子分泌的作用增强，经Caco-2细胞跨膜转运后共检测到24 条多肽序列，其中包括12 条四肽和5 条三肽，分子质量小于500 Da的小肽占62.50%；合成小肽PAG、LVG、LGV、PVL具有缓解巨噬细胞分泌NO、肿瘤坏死因子-α的功能。因此，模拟胃肠道消化可以改变火腿肽的组成，进而提升其抗炎活性；跨膜转运后的小肽具有缓解巨噬细胞炎症因子分泌的功能。

Effects of Gastrointestinal Digestion In Vitro and Transmembrane Transport on Anti-Inflammatory Activity of Bioactive Peptides from Dry-Cured Ham

The changes in the sequence and anti-inflammatory properties of bioactive peptides extracted from Xuanwei ham were analyzed after stimulated gastrointestinal digestion in vitro and transport across Caco-2 monolayers. The results showed that the total number of bioactive peptides increased after gastrointestinal digestion, and the proportion of peptides with a molecular mass below 1 000 Da in the total number of peptides increased from 40.18% to 57.40%. The inhibitory effect of the peptides on the secretion of inflammatory cytokines in RAW264.7 macrophages was enhanced after simulated digestion. After transport across Caco-2 cells, a total of 24 peptides were detected, including 12 tetrapeptides and 5 tripeptides, of which 62.50% were peptides with a molecular mass less than 500 Da. The synthetic peptides PAG, LVG, LGV and PVL were found to be able to suppress the secretion of NO and tumor necrosis factor α in macrophages. In conclusion, simulated gastrointestinal digestion can change the composition of the peptides from ham and in turn increase the anti-inflammatory activity. In addition, the small peptides transported across Caco-2 cells can inhibit the secretion of inflammatory factors in RAW264.7 macrophages.