饲喂仿刺参性腺对小鼠免疫功能的调节作用

以仿刺参雌、雄性腺冻干粉为原料,对其基本营养组分和主要功效成分进行测定,采用小鼠饲喂实验,考察仿刺参性腺在小鼠免疫功能调节方面的作用。结果表明:仿刺参雌、雄性腺富含营养和功效成分,其中雄性性腺蛋白质含量高达74.54 g/100 g,显著高于仿刺参体壁(P<0.05),雌性性腺中海参多糖含量与体壁相当,而海参皂苷含量高达4.77 g/100 g,是体壁的10倍以上;与对照组相比,饲喂仿刺参性腺可显著增加小鼠的胸腺指数和脾脏指数,提高自然杀伤细胞活性,尤其是饲喂雄性性腺的小鼠,自然杀伤细胞活性率升高到(47.19±4.99)%,血清中白细胞介素-6含量为(209.75±24.82) pg/mL,血清和肝脏中乳酸脱氢酶、超氧化物歧化酶活力均有所提升,肝脏中丙二醛含量降低至(1.98±0.25) nmol/mg。由此可知,饲喂仿刺参性腺可提高小鼠的免疫机能,且饲喂雄性性腺的作用更加明显。

Effects of Feeding Apostichopus japonicus Gonads on Immune Modulation in Mice

The basic nutritional and major functional components of the lyophilized powder of Apostiopsis japonicus gonads were determined. The effects of Apostiopsis japonicus gonads on immune function in mice were also investigated. Results showed that both the female and male gonads were rich in nutrients and functional components. The protein content of the male gonad was as high as 74.54 g/100 g, which was significantly higher than that of the body wall of Apostiopsis japonicus (P < 0.05). The polysaccharide content of the female gonad was comparable to that of the body wall. The saponin content was as high as 4.77 g/100 g, which was more than ten times higher than that of the body wall. Compared with the control group, feeding Apostiopsis japonicus gonads could significantly increase the thymus and spleen indices of mice and improve the activity of natural killer (NK) cell. In the mice fed with the male gonad, the NK cell activity increased to (47.19 ± 4.99)%, the serum level of interleukin-6 (IL-6) was (209.75 ± 24.82) pg/mL, the activities of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) in serum and liver increased, and the content of malondialdehyde (MDA) in liver decreased to (1.98 ± 0.25) nmol/mg. In conclusion, feeding Apostiopsis japonicus gonads could improve immune function in mice, and the effect of the male gonad was more pronounced.