食品中金黄色葡萄球菌定量检测方法比较研究

目的：研究食品中金黄色葡萄球菌定量检测方法。方法：比较Baird—Parker平板、显色培养基A、显色培养基B、Petrifilm TM测试片等4种计数方法的生长率、选择性、检出限及人工污染食品的检验效果。结果：金黄色葡萄球菌在显色培养基A上的生长率最高，PetrifilmTM测试片的选择性最强，4种计数方法的检出限相当，检出结果与实际含菌量无显著差异。结论：选择显色培养基作为日常检验的辅助手段可大大提高检测灵敏度、缩短检验周期、节约检验成本，值得在日常检验中推广应用。

Comparison of methods in quantitative detection of Staphylococcus aureus in food

Objective：To research the methods in quantitative detection of Staphylococcus aureus in food.Method： Compare four methods including Baird - Parker tablet, chrombgenie medium A, chromogenic medium B, PetrifilmTM test paper by growth rate, selectivity, detection limit and the effect of Contaminated foods in spection. Results： Chromogenie medium A had highest growth rate, PetrifilmTM test paper had best selectivity,the four methods that had no significant difference on de- tection results with the true value had similar detection limits. Conclusion：Chromogenic medium as an auxiliary means of daily inspection is worthy of popularization and application, it may improve the detection sensitivity, shorten testing cycle, saving the cost of inspection.