| 高效液相色谱法测定乳制品中的叶黄素 |
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| 引用本文： | 高迎春,尹伶灵,陈玲,陆庆泉,牛华星,张呈军,魏秀丽.高效液相色谱法测定乳制品中的叶黄素[J].中国乳品工业,2012,40(4):48-50. |
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| 作者姓名： | 高迎春 尹伶灵 陈玲 陆庆泉 牛华星 张呈军 魏秀丽 |
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| 作者单位： | 山东省兽药质量检验所,济南,250022 |
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| 摘 要： | 建立了一种简单、快速、可靠的测定乳制品中叶黄素的新方法。乳制品中的叶黄素经正己烷提取,氮气吹至近干,残余物用正己烷-乙酸乙酯(70+30)溶解后,高效液相色谱法测定,标准曲线法定量。该方法具有良好的准确度和精密度,在不同乳制品中添加回收率范围为91.0%～102.6%,相对标准偏差(RSD)为0.64～2.92。该方法的检出限和最低定量限分别为20μg/kg和50μg/kg。
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| 关 键 词： | 乳制品 叶黄素 高效液相色谱法 |
Determination of lutein in dairy products by high performance liquid chromatography |
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| GAO Ying-chun
,
YIN Ling-ling
,
CHEN Ling
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LU Qing-quan
,
NIU Hua-xing
,
ZHANG Cheng-jun
,
WEI Xiu-li.Determination of lutein in dairy products by high performance liquid chromatography[J].China Dairy Industry,2012,40(4):48-50. |
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| Authors: | GAO Ying-chun YIN Ling-ling CHEN Ling LU Qing-quan NIU Hua-xing ZHANG Cheng-jun WEI Xiu-li |
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| Affiliation: | (Shandong Provincial Veterinary Medicine,Jinan 250022,China) |
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| Abstract: | A simple fast and reliable method has been developed for the determination of lutein in dairy products.The samples were extracted by n-hexane,blown nearly dry with nitrogen.The residue was dissolved by n-hexane-ethyl acetate(70+30),and the final solution was determined by HPLC.The method was quantified by calibration curve method.The method has good accuracy and precision.The recovery of lutein in dairy products was 91.0%～102.6%.The relative standard deviations(RSD) was 0.64～2.92.The detection limits of lutein was 20 μg/kg,and the quantification limits was 50 μg/kg. |
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| Keywords: | dairy products lutein high performance liquid chromatography |
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