In vitro evaluation of poly(caporlactone) grafted dextran (PGD) nanoparticles with cancer cell |
| |
Authors: | P. Prabu Atul A. Chaudhari Santosh Aryal N. Dharmaraj S. Y. Park W. D. Kim H. Y. Kim |
| |
Affiliation: | (1) Department of Bionanosystem Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea;(2) College of Veterinary Medicine, Chonbuk National University, Jeonju, 561-756, Republic of Korea;(3) Center for Healthcare Technology Development, Chonbuk National University, Jeonju, 561-756, Republic of Korea;(4) Department of Chemistry, Bharathiar University, Coimbatore, 641 046, India;(5) Department of Future Technology, Korea Institute of Machinery and Materials, 171 Jang-dong, Yuseong-gu, Daejeon, 305-343, Republic of Korea;(6) Department of Textile Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea |
| |
Abstract: | This study dealt with the preparation and characterization of coumarin-6 loaded poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) and evaluation of cellular uptake by using human gastric cancer cell line (SNU-638), in vitro. The potential application of these PGD NPs for sustained drug delivery was evaluated by the quantification and localization of the cellular uptake of fluorescent PGD NPs. Coumarin-6 loaded PGD NPs were prepared by a modified oil/water emulsion technique and characterized by various physico-chemical methods such as, laser light scattering for particle size and size distribution, atomic force microscopy (AFM), zeta-potential and spectrofluorometry to identify the release of fluorescent molecules from the NPs. SNU-638 was used to measure the cellular uptake of fluorescent PGD NPs. Confocal laser scanning microscopic images clearly showed the internalization of NPs by the SNU-638 cells. Cell viability was assessed by treating the SNU-638 cells with PGD NPs for 48 h. The results reveal, that these biodegradable polymeric NPs holds promise in biomedical field as a carrier. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|