| 硬脂酰辅酶A去饱和酶1基因表达对草原安格斯牛血液脂肪酸组成的影响 |
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| 引用本文： | 王圆圆,李新淼,HESHUOTE Mailisi,白玉廷,包音都古荣·金花,呼格吉勒图,侯荣伦,薛强,敖日格勒. 硬脂酰辅酶A去饱和酶1基因表达对草原安格斯牛血液脂肪酸组成的影响[J]. 肉类研究, 2022, 36(2): 9-14. DOI: 10.7506/rlyj1001-8123-20210603-168 |
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| 作者姓名： | 王圆圆 李新淼 HESHUOTE Mailisi 白玉廷 包音都古荣·金花 呼格吉勒图 侯荣伦 薛强 敖日格勒 |
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| 作者单位： | 内蒙古农业大学食品科学与工程学院,内蒙古 呼和浩特 010018;内蒙古自治区生物制造重点实验室,内蒙古 呼和浩特 010018;哥伦比亚大学工程与应用科学学院,美国 纽约 10027;内蒙古农业大学兽医学院,内蒙古 呼和浩特 010018;内蒙古贺斯格绿色产业进出口有限公司,内蒙古 锡林郭勒 026321 |
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| 基金项目： | 内蒙古自然科学基金项目（2017MS0357）;;内蒙古自治区科技计划项目（201803055）; |
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| 摘 要： | 研究硬脂酰辅酶A去饱和酶1(stearoyl-coenzyme A desaturase 1,SCD1)基因表达对草原安格斯牛血液脂肪酸组成的影响,采集38头平均体质量(698±34)?kg、48月龄草原安格斯牛血液样品,测定其脂肪酸组成与含量,采用实时荧光定量聚合酶链式反应测定SCD1基因表达量,一代测序技术检测SC...
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| 关 键 词： | 草原安格斯牛 血液脂肪酸组成 硬脂酰辅酶A去饱和酶1基因表达量 相关性 硬脂酰辅酶A去饱和酶1基因分型 |
Effects of Stearoyl-Coenzyme A Desaturease 1 Gene Expression on Blood Fatty Acid Composition of Pastured Angus Cattle |
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| WANG Yuanyuan,LI Xinmiao,HESHUOTE Mailisi,BAI Yuting,BAOYINDUGURONG·Jinhua,HUGEJILETU,HOU Ronglun,XUE Qiang,AORIGELE. Effects of Stearoyl-Coenzyme A Desaturease 1 Gene Expression on Blood Fatty Acid Composition of Pastured Angus Cattle[J]. Meat Research, 2022, 36(2): 9-14. DOI: 10.7506/rlyj1001-8123-20210603-168 |
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| Authors: | WANG Yuanyuan LI Xinmiao HESHUOTE Mailisi BAI Yuting BAOYINDUGURONG·Jinhua HUGEJILETU HOU Ronglun XUE Qiang AORIGELE |
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| Affiliation: | 1.College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Inner Mongolia Key Laboratory of Biomanufacturing, Hohhot 010018, China; 3.College of Engineering and Applied Sciences, University of Columbia, New York 10027, USA; 4.College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China; 5.Inner Mongolia Hesige Green Industry Import and Export Co. Ltd., Xilingol 026321, China |
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| Abstract: | The purpose of this study was to investigate the effect of stearoyl-coenzyme desaturase 1 (SCD1) gene expression on blood fatty acid composition of Angus cattle raised on pasture. Blood samples of 38 pastured Angus cattle with average body mass of (698 ± 34) kg and at the age of 48 months were collected for fatty acid composition and content determination. The expression of the SCD1 gene was detected by real-time fluorescence quantitative polymerase chain reaction, and the influence of SCD1 gene mutation at C878T on fatty acid composition of blood samples of cattle was detected by firstgeneration sequencing technology. The correlation between SCD1 gene expression and fatty acid composition was further analyzed. The results showed the presence of 36 main fatty acids in blood samples, with the major saturated fatty acids (SFA) being palmitic acid (C16:0, 22.53%) and stearic acid (C18:0, 26.95%). Oleic acid (C18:1 n-9c) was identified as the most abundant monounsaturated fatty acid (MUFA) (15.09%). The significance analysis indicated that SCD1 gene expression had significant effects on myristic acid (C14:0), myristic acid (C14:1), C16:0, C16:1, C18:0, C18:1 n-9c, linoleic acid (C18:2 n-6c), MUFA and MUFA/SFA ratio as well as C14 index, C18 index, total unsaturation index and elongation index of fatty acids. Additionally, the correlation analysis results suggested that SCD1 gene expression was positively correlated with C16:0, palmitoleic acid (C16:1), C18:0, C18:1 n-9c and MUFA. SCD1 gene exhibited C/T mutation at the 878 site, yielding three genotypes: CC, TT and CT. No significant difference between different genotypes and fatty acid composition was found. Overall, the expression of SCD1 gene in the blood of pastured Angus cattle can regulate its fatty acid composition and thus can be used as a reference gene for genetic markers. |
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| Keywords: | pastured Angus cattle blood fatty acid composition stearoyl-coenzyme A desaturase 1 gene expression level correlation stearoyl-coenzyme A desaturase 1 genotype |
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