动物源性食品中甲基睾酮残留检测方法研究进展

甲基睾酮是一种激素类药物，在畜牧业和水产养殖业中作为动物促生长剂和性别逆转剂而被使用。我国农业农村部235号公告规定了在所有动物组织中不得检出甲基睾酮，但是目前还存在违禁使用的情况，因此有必要对其残留量进行检测。本文主要概述了甲基睾酮的作用机制、危害和法规标准，系统综述了现有畜禽、水产品中甲基睾酮残留检测常用的样品前处理方法和分析方法。样品前处理方法的确定包括提取试剂的选择、净化方法的优化等，检测方法主要包括高效液相色谱法、液相色谱-串联质谱法、液相色谱/质谱联用法和免疫分析方法等。本文分析了不同检测方法的优缺点、适用范围以及选择依据，并对检测方法进行了展望，旨在为加强动物源性食品中甲基睾酮的残留监测提供参考。

Research progress on the detection methods of methyltestosterone residues in animal-derived foods

Methyltestosterone is a kind of hormone drug, which is used as growth promoters and sex reversal agents in the aquaculture and livestock industry. In China the Ministry of Agriculture rural affairs Bulletin No. 235 stipulates that methyltestosterone cannot be detected in all animal tissues.. However, there are still cases of illegal use, so it is necessary to detect methyltestosterone residues. This paper mainly summarizes the action mechanism, harm, regulations and regulatory standards of methyltestosterone, systematically describes the existing sample pretreatment methods and analysis methods of methyltestosterone residue detection in livestock and aquatic species. Determination of sample pretreatment methods include the selection of extraction reagents, optimization of purification methods and etc, the detection methods of methyltestosterone residues in animal-derived foods mainly include high performance liquid chromatography, liquid chromatography-tandem mass spectrometry, liquid chromatography/mass spectrometry and immunoassay etc. Meanwhile, the paper analyzes the application scope, advantages and disadvantages and selection basis of different detection methods, and looks forward to the detection methods, to provide reference for strengthening the monitoring of methyltestosterone residues in animal-derived food.