保活运输过程中武昌鱼肌肉品质的变化

以武昌鱼为原料，采用超高效液相色谱、色彩色差计、紫外分光光度计等技术手段测定有水运输过程中（3、6、12、24 h）武昌鱼肌肉品质的变化。结果表明：运输3 h的武昌鱼pH值、持水性、白度（W）、亮度值（L*）、红度值（a*）、乳酸含量、肌糖原含量、5’-三磷酸腺苷（ATP）及其代谢关联产物总含量分别为6.57、70.33%、52.22、52.27、0.29、4.50 mg/g、1.46 mg/g、909.25 mg/100 g；随着运输时间延长至24 h，pH值先显著上升后显著下降到6.33（P＜0.05），乳酸含量先显著下降然后上升到3.81 mg/g，持水性逐渐上升到76.05%再下降至73.33%，W和L*逐渐下降至47.64和47.71，而a*显著升高至1.31（P＜0.05），肌糖原含量逐渐下降至1.25 mg/g，ATP及其代谢关联产物总含量上升至1 116.62 mg/100 g，其中ATP含量下降至62.43 mg/100 g，鲜味物质5’-次黄嘌呤核苷酸含量上升至747.91 mg/100 g，苦味物质次黄嘌呤核苷和次黄嘌呤含量分别上升至156.12、63.28 mg/100 g。以上研究结果表明，受应激影响，武昌鱼在保活运输中肌肉品质随时间显著改变。

Changes in Muscle Quality of Wuchang Bream during Live Transportation

The changes in the muscle quality of Wuchang bream during live transportation (3, 6, 12 and 24 h) were measured by ultra-high performance liquid chromatography, a color difference meter and an ultraviolet spectrophotometer. The results showed that after 3 h of transportation, the pH value, water-holding capacity, whiteness, brightness (L*), redness (a*), and the contents of lactic acid, muscle glycogen, ATP and its derivatives in fish muscle were 6.57, 70.33%, 52.22, 52.27, 0.29, 4.50 mg/g, 1.46 mg/g and 909.25 mg/100 g, respectively. With increasing transportation time to 24 h, the pH value first increased and then decreased to 6.33 (P < 0.05), while the lactic acid content initially decreased and then increased to 3.81 mg/g (P < 0.05). The water-holding capacity gradually increased to 76.05% and then decreased to 73.33%. The whiteness and L* gradually decreased to 47.64 and 47.71, respectively, whereas a* increased to 1.31 (P < 0.05). The content of muscle glycogen gradually decreased to 1.25 mg/g. The total content of ATP and its derivatives increased to 1 116.62 mg/100 g; the ATP content decreased to 62.43 mg/100 g, while the content of the umami substance IMP increased to 747.91 mg/100 g, and the contents of the bitter substances inosine (HxR) and hypoxanthine (Hx) increased to 156.12 and 63.28 mg/100 g, respectively. It was concluded that the muscle quality of Wuchang bream changed significantly during live transportation under the influence of stress.